蔗糖信号通过 MAPK 级联调控. 中的花色素苷生物合成。
Sucrose Signaling Regulates Anthocyanin Biosynthesis Through a MAPK Cascade in .
机构信息
The Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, People's Republic of China
The Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, People's Republic of China.
出版信息
Genetics. 2018 Oct;210(2):607-619. doi: 10.1534/genetics.118.301470. Epub 2018 Aug 16.
Abstract
Anthocyanin accumulation specifically depends on sucrose (Suc) signaling. However, the molecular basis of this process remains unknown. In this study, pull-down assays identified ETHYLENE-INSENSITIVE3 (EIN3), a component of both sugar signaling or/and metabolism. This protein interacted with YDA, and the physiological relevance of this interaction was confirmed by co-immunoprecipitation, yeast two-hybrid (Y2H) assay, and bimolecular fluorescence complementation. () double-mutant seedlings, but not seedlings, showed anthocyanin accumulation. Furthermore, suppressed anthocyanin accumulation in plants. Thus, may form a sugar-mediated gene cascade integral to the regulation of anthocyanin accumulation. Moreover, the gene cascade module directly targeted the promoter of () by direct EIN3 binding. Collectively, our data inferred a molecular model where the signaling cascade of the YDA-EIN3-TT8 appeared to target via EIN3, thereby modulating Suc signaling-mediated anthocyanin accumulation.
摘要
花青苷的积累取决于蔗糖（Suc）信号。然而，这个过程的分子基础仍然未知。在这项研究中，下拉实验鉴定出 ETHYLENE-INSENSITIVE3（EIN3），它是糖信号或/和代谢的组成部分。该蛋白与 YDA 相互作用，这种相互作用的生理相关性通过共免疫沉淀、酵母双杂交（Y2H）测定和双分子荧光互补得到证实。（）双突变体幼苗，而不是突变体幼苗，表现出花青苷的积累。此外，在 植株中抑制花青苷的积累。因此， 可能形成一个糖介导的基因级联，整合到花青苷积累的调控中。此外，基因级联模块通过直接 EIN3 结合直接靶向（）的启动子。总的来说，我们的数据推断出一个分子模型，其中 YDA-EIN3-TT8 的信号级联似乎通过 EIN3 靶向 ，从而调节 Suc 信号介导的花青苷积累。
相似文献
1
Sucrose Signaling Regulates Anthocyanin Biosynthesis Through a MAPK Cascade in .蔗糖信号通过 MAPK 级联调控. 中的花色素苷生物合成。
Genetics. 2018 Oct;210(2):607-619. doi: 10.1534/genetics.118.301470. Epub 2018 Aug 16.
2
Integration of Environmental and Developmental (or Metabolic) Control of Seed Mass by Sugar and Ethylene Metabolisms in Arabidopsis.通过阿拉伯idopsis 中的糖和乙烯代谢来整合环境和发育（或代谢）对种子质量的控制。
J Agric Food Chem. 2018 Apr 4;66(13):3477-3488. doi: 10.1021/acs.jafc.7b05992. Epub 2018 Mar 20.
3
ETHYLENE INSENSITIVE3 and ETHYLENE INSENSITIVE3-LIKE1 repress SALICYLIC ACID INDUCTION DEFICIENT2 expression to negatively regulate plant innate immunity in Arabidopsis.乙烯不敏感3和乙烯不敏感3样1抑制水杨酸诱导缺陷2的表达，从而对拟南芥的植物固有免疫进行负调控。
Plant Cell. 2009 Aug;21(8):2527-40. doi: 10.1105/tpc.108.065193. Epub 2009 Aug 28.
4
The Arabidopsis EIN3 binding F-Box proteins EBF1 and EBF2 have distinct but overlapping roles in ethylene signaling.拟南芥EIN3结合F-Box蛋白EBF1和EBF2在乙烯信号传导中具有不同但重叠的作用。
Plant Cell. 2007 Feb;19(2):509-23. doi: 10.1105/tpc.106.048140. Epub 2007 Feb 16.
5
Ethylene-induced stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 is mediated by proteasomal degradation of EIN3 binding F-box 1 and 2 that requires EIN2 in Arabidopsis.乙烯诱导的 ETHYLENE INSENSITIVE3 和 EIN3-LIKE1 的稳定是通过泛素蛋白酶体途径降解 EIN3 结合 F-box 1 和 2 来实现的，该过程需要拟南芥中的 EIN2。
Plant Cell. 2010 Jul;22(7):2384-401. doi: 10.1105/tpc.110.076588. Epub 2010 Jul 20.
6
Interaction between the bHLH transcription factor FIT and ETHYLENE INSENSITIVE3/ETHYLENE INSENSITIVE3-LIKE1 reveals molecular linkage between the regulation of iron acquisition and ethylene signaling in Arabidopsis.FIT 与 ETHYLENE INSENSITIVE3/ETHYLENE INSENSITIVE3-LIKE1 转录因子的相互作用揭示了拟南芥中铁摄取调控和乙烯信号转导之间的分子联系。
Plant Cell. 2011 May;23(5):1815-29. doi: 10.1105/tpc.111.084715. Epub 2011 May 17.
7
The - Gene Cascade Induces Anthocyanin Accumulation by Regulating Sucrose Levels.该基因级联通过调节蔗糖水平诱导花青素积累。
Front Plant Sci. 2016 Nov 22;7:1728. doi: 10.3389/fpls.2016.01728. eCollection 2016.
8
Arabidopsis ROOT HAIR DEFECTIVE3 is involved in nitrogen starvation-induced anthocyanin accumulation.拟南芥 ROOT HAIR DEFECTIVE3 参与氮饥饿诱导的花色素苷积累。
J Integr Plant Biol. 2015 Aug;57(8):708-21. doi: 10.1111/jipb.12320. Epub 2015 Feb 2.
9
EIN3 and ORE1 Accelerate Degreening during Ethylene-Mediated Leaf Senescence by Directly Activating Chlorophyll Catabolic Genes in Arabidopsis.EIN3和ORE1通过直接激活拟南芥中的叶绿素分解代谢基因，在乙烯介导的叶片衰老过程中加速叶片变黄。
PLoS Genet. 2015 Jul 28;11(7):e1005399. doi: 10.1371/journal.pgen.1005399. eCollection 2015 Jul.
10
An AP2 domain-containing gene, ESE1, targeted by the ethylene signaling component EIN3 is important for the salt response in Arabidopsis.一个含有 AP2 结构域的基因 ESE1，是乙烯信号组分 EIN3 的靶标，对拟南芥的盐响应很重要。
Plant Physiol. 2011 Oct;157(2):854-65. doi: 10.1104/pp.111.179028. Epub 2011 Aug 10.
引用本文的文献
1
Mutations in the floral regulator gene HUA2 restore flowering to the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant.花调控基因HUA2中的突变可使拟南芥海藻糖6-磷酸合酶1（tps1）突变体恢复开花。
Plant Physiol. 2025 May 30;198(2). doi: 10.1093/plphys/kiaf225.
2
Ethylene promotes anthocyanin synthesis in 'Viviana' lily via the LvMYB5-LvERF113-LvMYB1 module.乙烯通过LvMYB5-LvERF113-LvMYB1模块促进‘维维安娜’百合中的花青素合成。
Hortic Res. 2025 Feb 25;12(6):uhaf059. doi: 10.1093/hr/uhaf059. eCollection 2025 Jun.
3
Integrating physiological and anatomical insights to unveil the mechanism of coloration in Prunus sibirica.整合生理和解剖学见解以揭示西伯利亚李的着色机制。
Sci Rep. 2025 Feb 28;15(1):7115. doi: 10.1038/s41598-025-91280-w.
4
Trichome development of systemic developing leaves is regulated by a nutrient sensor-relay mechanism within mature leaves.系统发育叶片的表皮毛发育受成熟叶片内营养传感器-传递机制调控。
Sci Adv. 2025 Feb 7;11(6):eadq5820. doi: 10.1126/sciadv.adq5820. Epub 2025 Feb 5.
5
Identification and functional characterization of AsWRKY9, a WRKY transcription factor modulating alliin biosynthesis in garlic (Allium sativum L.).大蒜（Allium sativum L.）中调控蒜氨酸生物合成的WRKY转录因子AsWRKY9的鉴定与功能表征
BMC Biol. 2025 Jan 13;23(1):14. doi: 10.1186/s12915-025-02116-y.
6
CsWRKY29, a key transcription factor in tea plant for freezing tolerance, ABA sensitivity, and sugar metabolism.CsWRKY29，茶树中与抗寒性、ABA 敏感性和糖代谢相关的关键转录因子。
Sci Rep. 2024 Nov 19;14(1):28620. doi: 10.1038/s41598-024-80143-5.
7
transcription factor inhibits tanshinone accumulation in response to ethylene signaling in .转录因子抑制丹参酮在响应乙烯信号时的积累 。 （注：原文句子不完整，推测补充完整后翻译，原句中“in.”应是有遗漏的内容）
Front Plant Sci. 2024 Apr 2;15:1356922. doi: 10.3389/fpls.2024.1356922. eCollection 2024.
8
RHA2b-mediated MYB30 degradation facilitates MYB75-regulated, sucrose-induced anthocyanin biosynthesis in Arabidopsis seedlings.RHA2b介导的MYB30降解促进了拟南芥幼苗中MYB75调控的、蔗糖诱导的花青素生物合成。
Plant Commun. 2024 Mar 11;5(3):100744. doi: 10.1016/j.xplc.2023.100744. Epub 2023 Nov 10.
9
Unleashing the Potential of EIL Transcription Factors in Enhancing Sweet Orange Resistance to Bacterial Pathologies: Genome-Wide Identification and Expression Profiling.释放 EIL 转录因子在提高甜橙对细菌性病害抗性中的潜力：全基因组鉴定和表达谱分析。
Int J Mol Sci. 2023 Aug 10;24(16):12644. doi: 10.3390/ijms241612644.
10
Sugar status in preexisting leaves determines systemic stomatal development within newly developing leaves.现存叶片的糖含量决定了新发育叶片中的系统气孔发育。
Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2302854120. doi: 10.1073/pnas.2302854120. Epub 2023 Jun 5.
本文引用的文献
1
Arabidopsis ANGUSTIFOLIA3 (AN3) is associated with the promoter of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) to regulate light-mediated stomatal development.拟南芥 ANGUSTIFOLIA3（AN3）与 CONSTITUTIVE PHOTOMORPHOGENIC1（COP1）启动子相关，以调节光介导的气孔发育。
Plant Cell Environ. 2018 Jul;41(7):1645-1656. doi: 10.1111/pce.13212. Epub 2018 May 10.
2
The - Gene Cascade Induces Anthocyanin Accumulation by Regulating Sucrose Levels.该基因级联通过调节蔗糖水平诱导花青素积累。
Front Plant Sci. 2016 Nov 22;7:1728. doi: 10.3389/fpls.2016.01728. eCollection 2016.
3
Mitogen-activated protein kinase kinase kinase (MAPKKK) 4 from rapeseed (Brassica napus L.) is a novel member inducing ROS accumulation and cell death.来自油菜（甘蓝型油菜）的丝裂原活化蛋白激酶激酶激酶（MAPKKK）4是诱导活性氧积累和细胞死亡的新成员。
Biochem Biophys Res Commun. 2015 Nov 27;467(4):792-7. doi: 10.1016/j.bbrc.2015.10.063. Epub 2015 Oct 20.
4
The ARF2-ANT-COR15A gene cascade regulates ABA-signaling-mediated resistance of large seeds to drought in Arabidopsis.ARF2-ANT-COR15A基因级联调控拟南芥中脱落酸信号介导的大种子对干旱的抗性。
J Cell Sci. 2015 Nov 1;128(21):3922-32. doi: 10.1242/jcs.171207. Epub 2015 Sep 22.
5
Arabidopsis AINTEGUMENTA mediates salt tolerance by trans-repressing SCABP8.拟南芥AINTEGUMENTA通过反式抑制SCABP8介导耐盐性。
J Cell Sci. 2015 Aug 1;128(15):2919-27. doi: 10.1242/jcs.172072. Epub 2015 Jun 8.
6
Transcription co-activator Arabidopsis ANGUSTIFOLIA3 (AN3) regulates water-use efficiency and drought tolerance by modulating stomatal density and improving root architecture by the transrepression of YODA (YDA).转录共激活因子拟南芥 ANGUSTIFOLIA3（AN3）通过调节气孔密度和改善根系结构来调节水分利用效率和耐旱性，通过反式抑制 YODA（YDA）来实现。
Plant Biotechnol J. 2015 Sep;13(7):893-902. doi: 10.1111/pbi.12324. Epub 2015 Jan 20.
7
Transcription coactivator Arabidopsis ANGUSTIFOLIA3 modulates anthocyanin accumulation and light-induced root elongation through transrepression of Constitutive Photomorphogenic1.转录共激活因子拟南芥 ANGUSTIFOLIA3 通过反式阻遏组成型光形态建成 1 来调节花色素苷积累和光诱导的根伸长。
Plant Cell Environ. 2015 Apr;38(4):838-51. doi: 10.1111/pce.12456. Epub 2014 Dec 7.
8
Transcription factors, sucrose, and sucrose metabolic genes interact to regulate potato phenylpropanoid metabolism.转录因子、蔗糖和蔗糖代谢基因相互作用，以调控马铃薯苯丙烷类代谢。
J Exp Bot. 2013 Nov;64(16):5115-31. doi: 10.1093/jxb/ert303. Epub 2013 Oct 5.
9
Ethylene-insensitive3 is a senescence-associated gene that accelerates age-dependent leaf senescence by directly repressing miR164 transcription in Arabidopsis.乙烯不敏感3是一种衰老相关基因，它通过直接抑制拟南芥中miR164的转录来加速依赖年龄的叶片衰老。
Plant Cell. 2013 Sep;25(9):3311-28. doi: 10.1105/tpc.113.113340. Epub 2013 Sep 24.
10
Regulation of floral patterning and organ identity by Arabidopsis ERECTA-family receptor kinase genes.拟南芥 ERECTA 家族受体激酶基因对花形态建成和器官身份的调控。
J Exp Bot. 2013 Dec;64(17):5323-33. doi: 10.1093/jxb/ert270. Epub 2013 Sep 4.
Zotero 插件