Carreras-Planella Laura, Soler-Majoral Jordi, Rubio-Esteve Cristina, Lozano-Ramos Sara Inés, Franquesa Marcella, Bonet Josep, Troya-Saborido Maria Isabel, Borràs Francesc Enric
REMAR-IVECAT Group, "Germans Trias i Pujol" Health Science Research Institute, Can Ruti Campus, Badalona, Spain.
Department of Cell Biology, Physiology and Immunology, Autonomous University of Barcelona, Barcelona, Spain.
PLoS One. 2017 May 10;12(5):e0176987. doi: 10.1371/journal.pone.0176987. eCollection 2017.
Peritoneal Dialysis (PD) is considered the best option for a cost-effective mid-term dialysis in patients with Chronic Renal Failure. However, functional failure of the peritoneal membrane (PM) force many patients to stop PD treatment and start haemodialysis. Currently, PM functionality is monitored by the peritoneal equilibration test, a tedious technique that often show changes when the membrane damage is advanced. As in other pathologies, the identification and characterization of extracellular vesicles (EVs) in the peritoneal dialysis efflux (PDE) may represent a non-invasive alternative to identify biomarkers of membrane failure. Using size-exclusion chromatography, we isolated EVs from PDE in a group of patients. Vesicles were characterized by the presence of tetraspanin markers, nanoparticle tracking analysis profile, cryo-electron microscopy and mass spectrometry. Here, we report the isolation and characterization of PDE-EVs. Based on mass spectrometry, we have found a set of well-conserved proteins among patients. Interestingly, the peptide profile also revealed remarkable changes between newly enrolled and longer-treated PD patients. These results are the first step to the identification of PDE-EVs based new markers of PM damage, which could support clinicians in their decision-making in a non-invasive manner.
腹膜透析（PD）被认为是慢性肾衰竭患者进行具有成本效益的中期透析的最佳选择。然而，腹膜（PM）功能衰竭迫使许多患者停止PD治疗并开始血液透析。目前，PM功能通过腹膜平衡试验进行监测，这是一种繁琐的技术，当膜损伤进展时常常显示出变化。与其他病症一样，腹膜透析流出液（PDE）中细胞外囊泡（EVs）的鉴定和表征可能代表一种识别膜衰竭生物标志物的非侵入性替代方法。我们使用尺寸排阻色谱法从一组患者的PDE中分离出EVs。通过四跨膜蛋白标记物的存在、纳米颗粒跟踪分析图谱、冷冻电子显微镜和质谱对囊泡进行表征。在此，我们报告PDE-EVs的分离和表征。基于质谱分析，我们在患者中发现了一组保守性良好的蛋白质。有趣的是，肽谱还揭示了新入组和长期接受PD治疗的患者之间存在显著差异。这些结果是鉴定基于PDE-EVs的PM损伤新标志物的第一步，这可以以非侵入性方式支持临床医生进行决策。
