IPSBB Unit - Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Instituto de Catálisis y Petroleoquímica, CSIC, Calle de Marie Curie, 2, 28049 Madrid, Spain.
Food Chem. 2017 Oct 1;232:105-113. doi: 10.1016/j.foodchem.2017.03.149. Epub 2017 Mar 29.
A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae. The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan. A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components. The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations. The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
从子囊菌塔宾曲霉中获得了属于糖苷水解酶家族 11（GH11）的真菌内切木聚糖酶。对该酶进行了纯化、表征，并用于从桦木木聚糖生产木二糖混合物（XOS）。酶处理后获得了可观的中性 XOS 产率（28.8%），并且该混合物几乎不含木糖，主要成分是木二糖、木三糖和木四糖。通过分析母乳喂养儿童粪便发酵中微生物组成和有机酸谱的变化，证明了这种混合物具有益生元潜力。大量产生的乙酸和乳酸、潜在致病菌的减少以及双歧杆菌和可能有益的共生菌的增加，证实了这些木二糖的益生元价值。
