Lu Qi, Huang Xingjian, Lv Siyi, Pan Siyi
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
Food Chem. 2017 Oct 1;232:788-798. doi: 10.1016/j.foodchem.2017.04.064. Epub 2017 Apr 12.
Understanding the composition and content of carotenoids in red-flesh navel orange "Cara Cara" cultivated in different regions is valuable for its nutrition assessment and fruit processing control. Herein, the carotenoids of "Cara Cara" fruits, harvested from five typical citrus growing regions of China, were identified and quantified by HPLC-DAD coupled with HPLC-DAD-APCI-MS/MS. In saponified extracts, 19 free carotenoids including 8 carotenes and 11 xanthophylls were identified. In unsaponified extracts, 83.83-93.83% xanthophylls were esterified and 19 esters were inferred, with the 9-cis-violaxanthin ester confirmed as the dominant component. The carotenoid contents showed difference among the "Cara Cara" fruits from different growing regions, which might be attributed to the local environmental conditions, and the temperature was found directly correlated with total carotenoids content of "Cara Cara". The data obtained in this study will facilitate the nutritional evaluation of "Cara Cara" and provide beneficial guidance for fruit processing.
了解不同地区种植的红肉脐橙“卡拉卡拉”中类胡萝卜素的组成和含量，对其营养评估和果实加工控制具有重要价值。在此，采用高效液相色谱-二极管阵列检测联用高效液相色谱-二极管阵列-大气压化学电离质谱/质谱法，对采自中国五个典型柑橘种植区的“卡拉卡拉”果实中的类胡萝卜素进行了鉴定和定量分析。在皂化提取物中，鉴定出19种游离类胡萝卜素，包括8种胡萝卜素和11种叶黄素。在未皂化提取物中，83.83%-93.83%的叶黄素被酯化，推断出19种类胡萝卜素酯，其中9-顺式紫黄质酯被确认为主要成分。不同种植区的“卡拉卡拉”果实中类胡萝卜素含量存在差异，这可能归因于当地环境条件，且发现温度与“卡拉卡拉”果实的总类胡萝卜素含量直接相关。本研究获得的数据将有助于对“卡拉卡拉”进行营养评估，并为果实加工提供有益指导。
