Lou Yongli, Shi Jin, Guo Dewei, Qureshi Ahmad Kaleem, Song Laijun
The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Zhengzhou Central Hospital, Zhengzhou, Henan, China.
Saudi J Biol Sci. 2017 May;24(4):803-807. doi: 10.1016/j.sjbs.2015.06.025. Epub 2015 Jul 2.
Human glioma is a highly fatal tumor with a significant feature of immune suppression. The functions of PD-L1 refer to co-simulation and immune regulation. To investigate expression and functional activity of PD-L1 in human glioma cell in vivo and in vitro. Expressions of PD-L1mRNA and protein in the human glioma cell line were analyzed with quantitative RT-PCR and flow cytometer; and then expression of PD-L1 in tissue specimens of 10 glioma patients was treated with immunohistochemical analysis; glioma cell and allogeneic CD4 and CD8 T cells were co-cultured, and cytokine IFN-γ, IL-2 and IL-10 in cultured supernatant fluid were determined with ELISA; upon blocking the interaction between glioma cell and the immune cell with PD-L1 monoclonal antibody (5H1), surface markers on immune cells were analyzed using flow cytometer. ll human glioma cell lines constitutively expressed PD-L1, and IFN-γ induced glioma cell to highly express PD-L1. It was shown through immunohistochemical analysis that glioma specimen expressed PD-L1, while expression of PD-L1 was not observed in normal tissue and normal human brain near the tumor location. The release of IFN-γ and IL-2 was inhibited, while IL-10 was increased slightly. Glioma cell may escape from immune recognition and injury with the help of PD-L1, which is a significant pathogenic mechanism of glioma.
人类胶质瘤是一种具有显著免疫抑制特征的高度致命性肿瘤。程序性死亡配体1（PD-L1）的功能涉及共刺激和免疫调节。旨在研究PD-L1在人胶质瘤细胞体内和体外的表达及功能活性。采用定量逆转录聚合酶链反应（RT-PCR）和流式细胞仪分析人胶质瘤细胞系中PD-L1mRNA和蛋白的表达；然后用免疫组织化学分析法检测10例胶质瘤患者组织标本中PD-L1的表达；将胶质瘤细胞与同种异体CD4和CD8 T细胞共培养，并用酶联免疫吸附测定法（ELISA）测定培养上清液中的细胞因子γ干扰素（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-10（IL-10）；用PD-L1单克隆抗体（5H1）阻断胶质瘤细胞与免疫细胞之间的相互作用后，用流式细胞仪分析免疫细胞表面标志物。所有人类胶质瘤细胞系均组成性表达PD-L1，IFN-γ诱导胶质瘤细胞高表达PD-L1。免疫组织化学分析显示，胶质瘤标本表达PD-L1，而在肿瘤位置附近的正常组织和正常人脑中未观察到PD-L1的表达。IFN-γ和IL-2的释放受到抑制，而IL-10略有增加。胶质瘤细胞可能借助PD-L1逃避免疫识别和损伤，这是胶质瘤的一个重要致病机制。
