Daniels D L, Subbarao M N, Blattner F R, Lozeron H A
Department of Genetics, University of Wisconsin, Madison 53706.
Virology. 1988 Dec;167(2):568-77.
The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequences, 209 and 270 bp from the beginning of the late transcript, and lie on the stem of an inverted repeat which has features in common with previously described RNase III processing sites. This is the third example of RNase III cut sites immediately downstream of transcription termination points in lambda, the others being antiterminated N gene mRNA and int gene mRNA.
噬菌体λ体内Q激活的晚期基因RNA的RNA起始点位置已确定与体外6S RNA的起始点相同。6S RNA在感染早期产生，并被Q有效地抗终止。两个核糖核酸酶III切割位点位于Q依赖性RNA序列内，距晚期转录本起始处209和270 bp，位于一个反向重复序列的茎上，该序列具有与先前描述的核糖核酸酶III加工位点相同的特征。这是λ转录终止点下游紧邻核糖核酸酶III切割位点的第三个例子，其他的是抗终止的N基因mRNA和int基因mRNA。
