Tarannum Fouzia, Faizuddin Mohamed
Department of Periodontics, M. R. Ambedkar Dental College and Hospital, Karnataka, India.
Braz Dent J. 2017 Mar-Apr;28(2):140-147. doi: 10.1590/0103-6440201701094.
Lipoxins play an important role in periodontal resolution, hence, investigation of genetic polymorphism of lipoxin gene may provide important information on the role of lipoxins in periodontal disease pathogenesis. The aim of this study was to investigate a polymorphism of C-to-T substitution at position c.-292 in ALOX15 (reticulocyte-type 15 lipoxygenase 1) gene in patients with chronic periodontitis and to associate the polymorphism with gingival crevicular fluid (GCF) lipoxin A4 (LXA4) levels. Forty-five chronic periodontitis and 45 periodontally healthy patients were included in this case-control study. Plaque index, calculus index, sulcus bleeding index, full mouth probing depth (PD) and clinical attachment loss (CAL) were recorded. GCF and blood samples were collected. GCF was analyzed for LXA4 levels by enzyme linked immunosorbant assay. Genotyping of ALOX15 polymorphism was studied using PCR. Mean LXA4 was lower in periodontitis group compared to the periodontally healthy group. There was a negative correlation between CAL and LXA4. The CC genotype was higher in the study group than in the control group. In the study group, mean CAL was significantly lower among individuals with the CT genotype. Mean LXA4 was significantly lower in CC genotype (45.0±7.11 ng/mL) compared to CT genotype (50.81±5.81 ng/mL) among the patients with periodontitis. The results suggest that LXA4 and c.-292T allele are associated with periodontal health. Polymorphisms in the ALOX15 gene may influence periodontal disease pathogenesis. Hence, investigation of such polymorphisms could benefit the evaluation of lipoxins role in periodontal disease.
脂氧素在牙周炎症消退中发挥重要作用，因此，研究脂氧素基因的遗传多态性可能为脂氧素在牙周疾病发病机制中的作用提供重要信息。本研究的目的是调查慢性牙周炎患者中ALOX15（网织红细胞型15脂氧合酶1）基因c.-292位点C到T的替换多态性，并将该多态性与龈沟液（GCF）中脂氧素A4（LXA4）水平相关联。本病例对照研究纳入了45例慢性牙周炎患者和45例牙周健康患者。记录菌斑指数、牙石指数、龈沟出血指数、全口探诊深度（PD）和临床附着丧失（CAL）。采集GCF和血液样本。通过酶联免疫吸附测定法分析GCF中的LXA4水平。使用聚合酶链反应（PCR）研究ALOX15多态性的基因分型。与牙周健康组相比，牙周炎组的平均LXA4较低。CAL与LXA4之间存在负相关。研究组中CC基因型高于对照组。在研究组中，CT基因型个体的平均CAL显著较低。在牙周炎患者中，CC基因型的平均LXA4（45.0±7.11 ng/mL）显著低于CT基因型（50.81±5.81 ng/mL）。结果表明，LXA4和c.-292T等位基因与牙周健康相关。ALOX15基因的多态性可能影响牙周疾病的发病机制。因此，研究此类多态性可能有助于评估脂氧素在牙周疾病中的作用。
