Yücel O Ozer, Berker E, Mescil L, Eratalay K, Tepe E, Tezcan I
Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Genet Couns. 2013;24(1):21-35.
The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1beta (+3954) genotype and allele frequency in both chronic and aggressive periodontitis patients, and also to investigate whether this polymorphism is associated with gingival crevicular fluid (GCF) IL-1beta levels, periodontal disease severity and clinical parameters in subjects of Turkish origin.
A total of 147 individuals were enrolled in the study including 56 aggressive periodontitis (AP), 44 chronic periodontitis (CP) patients and 47 healthy controls (C). Single nucleotide polymorphism at IL-1beta (+3954) is analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). GCF samples were analyzed for IL-1beta, using enzyme linked immunosorbent assay (ELISA).
The distribution of genotypes and allele frequencies for IL-1beta (+3954) were similar among the groups, in spite of a trend toward a higher frequency of allele 2 in the patient groups. The genotype distribution and allele frequencies were also not different after stratification of subjects according to the clinical attachment level (CAL < 4 mm and CAL > 4mm). No differences were found between the GCF IL-1beta levels of the different genotypes. Allele 2 was associated with increased bleeding on probing (BOP) sites in chronic periodontitis patients.
The results of this study do not support that genetic polymorphism in the IL-1beta (+3954) could be identified as a susceptibility or severity factor in aggressive periodontitis, in the present population. The association of allele 2 frequency and higher percentage of BOP sites in chronic periodontitis suggest that IL-1beta (+3954) potentially play a significant but not major role in the clinical outcome.
调节白细胞介素-1β（IL-1β）基因表达、产生及生物活性的免疫机制和基因变异，被认为在牙周病发病机制中起重要作用。本研究旨在分析慢性和侵袭性牙周炎患者白细胞介素（IL）-1β（+3954）基因型和等位基因频率，并调查该多态性是否与土耳其裔受试者龈沟液（GCF）中IL-1β水平、牙周病严重程度及临床参数相关。
本研究共纳入147名个体，包括56例侵袭性牙周炎（AP）患者、44例慢性牙周炎（CP）患者和47名健康对照（C）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析IL-1β（+3954）的单核苷酸多态性。使用酶联免疫吸附测定（ELISA）分析GCF样本中的IL-1β。
尽管患者组中2号等位基因频率有升高趋势，但各组间IL-1β（+3954）基因型和等位基因频率分布相似。根据临床附着水平（CAL<4mm和CAL>4mm）对受试者进行分层后，基因型分布和等位基因频率也无差异。不同基因型的GCF IL-1β水平之间未发现差异。2号等位基因与慢性牙周炎患者探诊出血（BOP）部位增加有关。
本研究结果不支持在当前人群中，IL-1β（+3954）基因多态性可被视为侵袭性牙周炎的易感性或严重程度因素。慢性牙周炎中2号等位基因频率与较高比例的BOP部位相关，提示IL-1β（+3954）可能在临床结局中起重要但非主要作用。
