Tarannum F, Faizuddin M
Department of Periodontics, M.R. Ambedkar Dental College and Hospital, Bangalore, India.
J Periodontal Res. 2017 Apr;52(2):210-217. doi: 10.1111/jre.12383. Epub 2016 Apr 16.
The balance between host proinflammatory and anti-inflammatory immune responses is a key determinant for the pathogenesis of periodontal disease. This study aimed to explore the possibility of an association between lipoxin A4 and interleukin-12 in chronic periodontitis.
Forty-five patients with chronic periodontitis and 45 periodontally healthy patients were included in this case-control study. Plaque index, calculus index, gingival index, sulcus bleeding index, full-mouth probing depth and clinical attachment loss were recorded. Gingival crevicular fluid samples were collected and analysed for interleukin-12 and lipoxin A4 using ELISA.
The mean concentration of lipoxin A4 was lower in the periodontitis group compared with the periodontally healthy group. There was a negative correlation between interleukin-12 and lipoxin A4 in both groups. There was a negative correlation between clinical attachment loss and lipoxin A4, and a positive correlation between clinical attachment loss and interleukin-12. However, the correlations were statistically insignificant.
The mean interleukin-12 concentration was significantly higher in gingival crevicular fluid from patients with periodontitis than in that from with healthy patients, and the mean lipoxin A4 concentration was lower in patients with periodontitis than in healthy patients. Lipoxin A4 possibly has an inhibitory effect on interleukin-12.
宿主促炎和抗炎免疫反应之间的平衡是牙周病发病机制的关键决定因素。本研究旨在探讨脂氧素A4与白细胞介素-12在慢性牙周炎中存在关联的可能性。
本病例对照研究纳入了45例慢性牙周炎患者和45例牙周健康患者。记录菌斑指数、牙石指数、牙龈指数、龈沟出血指数、全口探诊深度和临床附着丧失情况。收集龈沟液样本，采用酶联免疫吸附测定法（ELISA）分析白细胞介素-12和脂氧素A4。
与牙周健康组相比，牙周炎组脂氧素A4的平均浓度较低。两组中白细胞介素-12与脂氧素A4均呈负相关。临床附着丧失与脂氧素A4呈负相关，与白细胞介素-12呈正相关。然而，这些相关性无统计学意义。
牙周炎患者龈沟液中白细胞介素-12的平均浓度显著高于健康患者，牙周炎患者脂氧素A4的平均浓度低于健康患者。脂氧素A4可能对白介素-12具有抑制作用。
