School of Mechanical and Material Engineering, Washington State University , Pullman, Washington 99164, United States.
School of Chemistry and Chemical Engineering, Southeast University , Nanjing, Jiangsu 211189, China.
ACS Appl Mater Interfaces. 2017 Jul 19;9(28):23325-23332. doi: 10.1021/acsami.6b15387. Epub 2017 Jul 3.
Sensitive assay and imaging of multiple low-abundance microRNAs (miRNAs) in living cells remain a grand challenge. Herein, based on polyelectrolyte-induced reduction, a facile approach has been proposed to synthesize novel MnO nanotubes. Owing to the remarkably strong fluorescence quenching ability, low cytotoxicity, and excellent colloid stability, the as-prepared MnO nanotubes showed great potential for simultaneous detection and imaging of multiple miRNAs in vitro and in situ in living cells for the first time. Besides, MnO nanotubes can be reduced to Mn by intracellular acid pH or glutathione, which may serve as an activatable contrast reagent for MRI. Therefore, the MnO nanotube-based probes, termed "NanoSearchlight", provide a promising, multimodal imaging tool for precise and accurate diagnosis and prognosis of cancers.
在活细胞中灵敏检测和成像多种低丰度 microRNA（miRNA）仍然是一个巨大的挑战。在此，基于聚电解质诱导的还原，提出了一种简便的方法来合成新型的 MnO 纳米管。由于具有显著的强荧光猝灭能力、低细胞毒性和优异的胶体稳定性，所制备的 MnO 纳米管首次显示出用于体外和原位同时检测和成像多种 miRNA 的巨大潜力。此外，MnO 纳米管可以被细胞内酸性 pH 值或谷胱甘肽还原为 Mn，这可能可用作 MRI 的激活对比试剂。因此，基于 MnO 纳米管的探针，称为“NanoSearchlight”，为癌症的精确和准确诊断及预后提供了一种很有前途的多模式成像工具。
