State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, PR China; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, PR China.
Institute for Materials Science and Devices, School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215011, PR China.
Anal Chim Acta. 2022 Mar 1;1196:339551. doi: 10.1016/j.aca.2022.339551. Epub 2022 Jan 25.
A novel type of core-shell "loading-type" nanomaterials, which integrated excellent biocompatibility, high loading capacity, efficient delivery, dual target recognition and response all-in-one, was fabricated for simultaneous imaging analysis of glutathione and microRNAs in living cells. Specifically, the core-shell "loading-type" nanomaterials (termed as MSNs@MnO) were formed with mesoporous silica nanoparticles (MSNs) as core and a two-dimensional manganese dioxide nanosheets (MnO) as outer layer. Based on the excellent loading capability, the core MSNs was utilized as carriers for signal molecules of rhodamine 6G (R6G). Meanwhile, the shell MnO acted as carriers for nucleic acid compounds, the locker for blocking R6G in the pore of MSNs, and reactant for reacting with redox species. Upon entering the cells, the specific redox reaction between the MnO nanosheets and cellular glutathione (GSH) induced the removal of the locker layer from the MSNs, thereby triggering unlocking, releasing, and recovering the corresponding fluorescence of R6G. While encounter with miRNAs, the molecular beacons (MB) adsorbed on the MnO nanosheets hybridized with target miRNA, which induced the conformational transition of the hairpin molecules, formed new secondary structures, and then recovered the fluorescence signal. Due to the each recovered fluorescence intensity was correlated with the corresponding target molecules, simultaneous detection of dual biomarkers was successfully achieved via the core-shell "loading-type" nanomaterials, which can provide more precise data guidance for diagnosis and disease treatment, and also own promising application in such research area.
一种新型的核壳“装载型”纳米材料，综合了优异的生物相容性、高载量、高效传递、双重靶向识别和响应于一体，被用于在活细胞中同时对谷胱甘肽和 microRNAs 进行成像分析。具体而言，核壳“装载型”纳米材料（称为 MSNs@MnO）由介孔硅纳米粒子（MSNs）作为核和二维二氧化锰纳米片（MnO）作为外层组成。基于优异的装载能力，核心 MSNs 被用作罗丹明 6G（R6G）信号分子的载体。同时，壳 MnO 作为核酸化合物的载体、MSNs 孔中 R6G 的锁定器以及与氧化还原物质反应的反应物。进入细胞后，MnO 纳米片与细胞内谷胱甘肽（GSH）之间的特定氧化还原反应导致锁定器层从 MSNs 上除去，从而触发解锁、释放和恢复相应的 R6G 荧光。当遇到 microRNAs 时，吸附在 MnO 纳米片上的分子信标（MB）与靶标 microRNA 杂交，诱导发夹分子的构象转变，形成新的二级结构，然后恢复荧光信号。由于每个恢复的荧光强度与相应的靶标分子相关，因此通过核壳“装载型”纳米材料成功实现了双重生物标志物的同时检测，这可为诊断和疾病治疗提供更精确的数据指导，并且在这类研究领域也具有广阔的应用前景。
