Caballero Silvia, Kim Sohn, Carter Rebecca A, Leiner Ingrid M, Sušac Bože, Miller Liza, Kim Grace J, Ling Lilan, Pamer Eric G
Immunology Program and Infectious Disease Service, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Immunology and Microbial Pathogenesis Program, Weill Cornell Graduate School of Medical Sciences, New York, NY 10065, USA.
Immunology Program and Infectious Disease Service, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell Host Microbe. 2017 May 10;21(5):592-602.e4. doi: 10.1016/j.chom.2017.04.002.
Antibiotic-mediated microbiota destruction and the consequent loss of colonization resistance can result in intestinal domination with vancomycin-resistant Enterococcus (VRE), leading to bloodstream infection in hospitalized patients. Clearance of VRE remains a challenging goal that, if achieved, would reduce systemic VRE infections and patient-to-patient transmission. Although obligate anaerobic commensal bacteria have been associated with colonization resistance to VRE, the specific bacterial species involved remain undefined. Herein, we demonstrate that a precisely defined consortium of commensal bacteria containing the Clostridium cluster XIVa species Blautia producta and Clostridium bolteae restores colonization resistance against VRE and clears VRE from the intestines of mice. While C. bolteae did not directly mediate VRE clearance, it enabled intestinal colonization with B. producta, which directly inhibited VRE growth. These findings suggest that therapeutic or prophylactic administration of defined bacterial consortia to individuals with compromised microbiota composition may reduce inter-patient transmission and intra-patient dissemination of highly antibiotic-resistant pathogens.
抗生素介导的微生物群破坏以及随之而来的定植抗性丧失，可导致耐万古霉素肠球菌（VRE）在肠道中占据主导地位，进而导致住院患者发生血流感染。清除VRE仍然是一个具有挑战性的目标，一旦实现，将减少全身性VRE感染以及患者之间的传播。虽然专性厌氧共生细菌与对VRE的定植抗性有关，但其中涉及的具体细菌种类仍不明确。在此，我们证明，一个精确界定的共生细菌联合体，其中包含梭菌属 XIVa 簇的普拉氏布劳特氏菌和博氏梭菌，可恢复对VRE的定植抗性，并从小鼠肠道中清除VRE。虽然博氏梭菌并未直接介导VRE的清除，但它能使普拉氏布劳特氏菌在肠道中定植，而普拉氏布劳特氏菌可直接抑制VRE的生长。这些发现表明，向微生物群组成受损的个体进行治疗性或预防性施用特定的细菌联合体，可能会减少高耐抗生素病原体在患者之间的传播以及在患者体内的扩散。
