一种高灵敏度的锌和汞荧光化学传感器及其应用。
A highly sensitive turn-on fluorescent chemosensor for recognition of Zn and Hg and applications.
机构信息
College of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
College of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Sep 5;184:177-183. doi: 10.1016/j.saa.2017.05.011. Epub 2017 May 7.
A fluorescence probe has been designed and synthesized, and applied with a combined theoretical and experimental study. Research suggests that the probe can be used to sense Zn and Hg through selective turn-on fluorescence responses in the aqueous HEPES buffer (0.05M, pH=7.4). The limit of detection (LOD) were determined as 1.46×10M (Zn) and 2.50×10M (Hg). Moreover, based on DFT, the geometry optimizations of probe 1, [1-Hg] complex and [1-Zn] complex were carried out using the Gaussian 09 program, in which the B3LYP function was used. The electronic properties of free probe 1 and the metal complexes were studied based on the Natural Bond Orbital (NBO) analyses. The probe 1 has also been successfully applied to detection of Zn and Hg in living cells.
设计并合成了一种荧光探针，并通过理论和实验相结合的方法进行了研究。研究表明，该探针可以通过在水相 HEPES 缓冲液（0.05M，pH=7.4）中选择性的开启荧光响应来检测 Zn 和 Hg。该探针的检测限（LOD）分别为 1.46×10M（Zn）和 2.50×10M（Hg）。此外，基于密度泛函理论（DFT），使用 Gaussian 09 程序对探针 1、[1-Hg]配合物和[1-Zn]配合物进行了几何优化，其中使用了 B3LYP 函数。根据自然键轨道（NBO）分析，研究了游离探针 1 和金属配合物的电子性质。该探针还成功地应用于活细胞中 Zn 和 Hg 的检测。
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