Cash P
Department of Bacteriology, University of Aberdeen, Foresterhill, Scotland.
Can J Microbiol. 1988 Jun;34(6):802-6. doi: 10.1139/m88-136.
High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of serotype 1 poliovirus were also analysed. The overall protein profile was conserved but the structural protein VP3 varied in its net charge among the viruses. Eight out of 14 clinical virus isolates had VP3 with a net basic charge identical to serotype 1 polio vaccine, whereas the remaining clinical isolates had an acidic VP3 similar to the nonvaccine type 1 strains. The altered VP3 mobility correlated with a change in antigenicity as determined by monoclonal antibodies directed to the neutralization site located on VP3. The data clearly illustrated the suitability of two-dimensional PAGE in analysing protein mutations in attenuated vaccine virus excreted by vaccinees.
高分辨率二维聚丙烯酰胺凝胶电泳用于分析1型脊髓灰质炎病毒分离株之间的蛋白质变异情况。将近期有口服减毒活脊髓灰质炎病毒疫苗史的患者所分离出的病毒与1型脊髓灰质炎疫苗原型进行比较。还对1型脊髓灰质炎病毒的非疫苗株Mahoney和Brunenders株进行了分析。整体蛋白质谱是保守的，但结构蛋白VP3在这些病毒中的净电荷有所不同。14株临床病毒分离株中有8株的VP3净电荷与1型脊髓灰质炎疫苗相同，而其余临床分离株的VP3呈酸性，类似于1型非疫苗株。如针对位于VP3上的中和位点的单克隆抗体所确定的，VP3迁移率的改变与抗原性变化相关。这些数据清楚地表明二维聚丙烯酰胺凝胶电泳适用于分析疫苗接种者排出的减毒疫苗病毒中的蛋白质突变。
