Yoneyama T, Hagiwara A, Hara M
Microbiol Immunol. 1981;25(6):575-83. doi: 10.1111/j.1348-0421.1981.tb00059.x.
Seventeen strains of type 2 poliovirus isolated from vaccines and vaccine-contacts in Japan were analyzed for viral structural proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. An alteration in electrophoretic mobility of VP1 was observed in most of the strains from paralytic patients and healthy vaccinees. The alteration in VP1 was observed at a high frequency among many strains, but no significant relation between the mobility change of VP1 and antigenic marker or rct/40 marker was observed. No alteration in mobility of other structural proteins was observed among the strains examined except for one with altered VP3. The results suggested that VP1 of Sabin type 2 strain was easily changeable during replication in human intestines.
对从日本疫苗及疫苗接触者中分离出的17株2型脊髓灰质炎病毒，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析其病毒结构蛋白。在大多数来自麻痹患者和健康疫苗接种者的毒株中，观察到VP1电泳迁移率发生改变。在许多毒株中，VP1改变的发生率较高，但未观察到VP1迁移率变化与抗原标记或rct/40标记之间存在显著关联。在所检测的毒株中，除一株VP3发生改变外，未观察到其他结构蛋白迁移率的改变。结果表明，2型萨宾株的VP1在人肠道复制过程中易于发生变化。
