Xue Dan, Chen Huai, Zhao Xinquan, Xu Shixiao, Hu Linyong, Xu Tianwei, Jiang Lin, Zhan Wei
Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041 Chengdu, China; College of Life Sciences, Sichuan University, 610065 Chengdu, China; University of Chinese Academy of Sciences, 100049 Beijing, China.
Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041 Chengdu, China.
Syst Appl Microbiol. 2017 Jun;40(4):227-236. doi: 10.1016/j.syapm.2017.03.006. Epub 2017 Apr 18.
Yak and Tibetan sheep are the major indigenous ruminants on the Qinghai-Tibetan Plateau in China. The aim of this work was to study the differences in ruminal fermentation parameters and rumen prokaryotic community composition between hosts and feeding paradigms. The 16S rRNA genes targeting bacteria and archaea were sequenced using the MiSeq platform. The results showed that the prokaryotic community structure between yak and Tibetan sheep was significantly different (P<0.01). A significant difference in structure was also found between groups of yaks barn fed with a total mixed ration (TMR) and those naturally grazing (NG) (P=0.034), as well as for Tibetan sheep of the two groups (P=0.026). The core prokaryotic populations that existed in the rumen mostly dominated the structure. There was an obvious correlation of the prokaryotic community composition at the phylum and genus levels with the host or the feeding pattern. In addition, Tibetan sheep showed significantly higher yields of volatile fatty acids (VFAs) than yak, as did the NG group compared with the TMR group. In conclusion, both the host and feeding pattern may influence rumen microbial ecology system, with host effects being more important than those of the feeding pattern.
牦牛和藏羊是中国青藏高原主要的本土反刍动物。本研究旨在探讨宿主与饲养方式对瘤胃发酵参数及瘤胃原核生物群落组成的影响。利用MiSeq平台对靶向细菌和古菌的16S rRNA基因进行测序。结果表明，牦牛和藏羊的原核生物群落结构存在显著差异（P<0.01）。舍饲全混合日粮（TMR）的牦牛组与自然放牧（NG）的牦牛组之间，以及两组藏羊之间，结构也存在显著差异（P=0.034和P=0.026）。瘤胃中存在的核心原核生物种群大多主导着群落结构。原核生物群落组成在门和属水平上与宿主或饲养方式存在明显的相关性。此外，藏羊的挥发性脂肪酸（VFA）产量显著高于牦牛，自然放牧组的VFA产量也高于TMR组。总之，宿主和饲养方式均可能影响瘤胃微生物生态系统，其中宿主效应比饲养方式的影响更为重要。
