Borges A, Lopez-Romero J C, Oliveira D, Giaouris E, Simões M
LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
CIAD, Research Center for Food and Development, Hermosillo, Sonora, Mexico.
J Appl Microbiol. 2017 Jul;123(1):104-115. doi: 10.1111/jam.13490.
The aim of this study was to investigate the antibiofilm potential of five essential oil (EO) components with cyclic (sabinene-SAB, carveol-C1, carvone-C2) and acyclic (citronellol-C3 and citronellal-C4) structures against Escherichia coli and Staphylococcus aureus.
The selected EO components prevented biofilm set-up, with C3 and C4 causing remarkable effects. When applied against pre-established biofilms, they promoted high biomass removal and inactivation of biofilm cells. Moreover, no viable E. coli biofilm cells were detected after exposure to SAB at 5 × MIC and 10 × MIC, and a significant viability decrease was observed for both bacteria with the other EO components. SAB, C3 and C4 caused the most prominent effects apparently due to their octanol-water partition coefficient (Po/w), the number of rotatable bonds (n-ROTB) and the free hydroxyl groups.
The overall results demonstrated that the selected EO components, particularly SAB, C3 and C4 are of interest as new lead molecules to both prevent biofilm set-up and to control pre-established biofilms of E. coli and S. aureus.
The tested EO components exhibited prominent antibiofilm properties against E. coli and S. aureus providing a novel and effective alternative/complementary approach to counteract chronic infections and the transmission of diseases in clinical settings.
本研究旨在调查五种具有环状（桧烯 - SAB、香芹醇 - C1、香芹酮 - C2）和无环（香茅醇 - C3和香茅醛 - C4）结构的精油（EO）成分对大肠杆菌和金黄色葡萄球菌的抗生物膜潜力。
所选的精油成分可防止生物膜形成，其中C3和C4产生显著效果。当作用于预先形成的生物膜时，它们能促进生物量的大量去除并使生物膜细胞失活。此外，在5×MIC和10×MIC的SAB处理后，未检测到存活的大肠杆菌生物膜细胞，并且其他精油成分处理后两种细菌的活力均显著下降。SAB、C3和C4产生最显著的效果显然是由于它们的正辛醇 - 水分配系数（Po/w）、可旋转键的数量（n - ROTB）和游离羟基。
总体结果表明，所选的精油成分，特别是SAB、C3和C4作为新型先导分子，在预防生物膜形成以及控制大肠杆菌和金黄色葡萄球菌预先形成的生物膜方面具有研究价值。
所测试的精油成分对大肠杆菌和金黄色葡萄球菌表现出显著的抗生物膜特性，为临床环境中对抗慢性感染和疾病传播提供了一种新颖且有效的替代/补充方法。
