Olszewska Magdalena A, Diez-Gonzalez Francisco
Center for Food Safety, College of Agricultural and Environmental Sciences, University of Georgia, Griffin, GA, United States.
Department of Industrial and Food Microbiology, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Front Microbiol. 2021 Jul 14;12:638933. doi: 10.3389/fmicb.2021.638933. eCollection 2021.
In nature, may interact competitively and cooperatively with other organisms, resulting in unique spatial organization and functions for cells within the community. This study was undertaken to characterize the biofilm architecture of binary biofilms of and species and to assess their effect on the survival of during exposure to hypochlorite. Three strains, ATCC 19115 (Lm5), ATCC 19117 (Lm7), and Coleslaw (LmC), were selected and combined individually with three strains: (Lf), (Lb), and (Lp). In binary Lm-Lp biofilms, the Lm cell counts were similar to single-species biofilms (8.5 log CFU/well), and the Lp cell numbers declined by 1.0 log CFU/well. In the presence of Lb, the Lm cell counts were reduced by 1.5 log CFU/well ( < 0.05), whereas the Lf cell counts increased at least by 3.5 log CFU/well. Confocal laser scanning microscopy (CLSM) determined that interspecies interactions significantly affected the spatial organization of three binary biofilms. Biofilm surface-to-volume ratio increased from 0.8 μm/μm for Lm5 in the monoculture to 2.1 μm/μm for Lm5-Lp in the dual-species model ( < 0.05), and was characterized by a thicker structure with a largely increased surface area. Biofilm roughness increased from 0.2 for Lm7 to 1.0 for Lm7-Lb biofilms ( < 0.05), which appeared as interspecific segregation. Biofilm thickness increased from 34.2 μm for LmC to 46.3 μm for LmC-Lf ( < 0.05), which produced flat and compact structures that covered the entire surface available. The biomass of the extracellular matrix was higher in the case of some binary biofilms ( < 0.05); however, this effect was dependent upon the species pair. When treated with hypochlorite, Lm5 in binary biofilms had an approximately 1.5 log CFU/well greater survival than individually. The unique spatial organization and greater protein production may explain the protective effect of Lp after hypochlorite exposure.
在自然界中，[具体生物]可能会与其他生物进行竞争和合作，从而形成群落内细胞独特的空间组织和功能。本研究旨在表征[具体生物A]和[具体生物B]二元生物膜的生物膜结构，并评估它们在次氯酸盐暴露期间对[具体生物A]存活的影响。选择了三株[具体生物A]菌株，即ATCC 19115（Lm5）、ATCC 19117（Lm7）和凉拌卷心菜（LmC），并分别与三株[具体生物B]菌株组合：[具体生物B1]（Lf）、[具体生物B2]（Lb）和[具体生物B3]（Lp）。在二元Lm - Lp生物膜中，Lm细胞计数与单物种生物膜相似（8.5 log CFU/孔），而Lp细胞数量每孔下降1.0 log CFU。在Lb存在的情况下，Lm细胞计数每孔减少1.5 log CFU（P < 0.05），而Lf细胞计数至少增加3.5 log CFU/孔。共聚焦激光扫描显微镜（CLSM）确定种间相互作用显著影响了三种二元生物膜的空间组织。生物膜的表面积与体积比从单培养中Lm5的0.8μm/μm增加到双物种模型中Lm5 - Lp的2.1μm/μm（P < 0.05），其特征是结构更厚，表面积大幅增加。生物膜粗糙度从Lm7的0.2增加到Lm7 - Lb生物膜的1.0（P < 0.05），表现为种间分离。生物膜厚度从LmC的34.2μm增加到LmC - Lf的46.3μm（P < 0.05），形成了覆盖整个可用表面的平坦且紧密的结构。在某些二元生物膜中，细胞外基质的生物量更高（P < 0.05）；然而，这种影响取决于物种对。用次氯酸盐处理时，二元生物膜中的Lm5比单独处理时每孔的存活率高约l.5 log CFU。独特的空间组织和更高的蛋白质产量可能解释了次氯酸盐暴露后Lp的保护作用。
