In Vitro Antibacterial and Antibiofilm Activity of Essential Oil, Citral, and Carvone against .

In vitro antimicrobial and antibiofilm activities of the essential oil and its major components (citral and carvone) against were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL when LA1EO and citral were used. Potential inhibition (100%) of biofilm formation at the concentration of 0.5 mg mL of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL, 2 mg mL, 2 mg mL, and 0.5 mg mL for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of EOs against , a species of recognized clinical interest.