Porfírio Emanuela Mesquita, Melo Hider Machado, Pereira Antônio Matheus Gomes, Cavalcante Theodora Thays Arruda, Gomes Geovany Amorim, de Carvalho Mário Geraldo, Costa Renata Albuquerque, Júnior Francisco Eduardo Aragão Catunda
INTA College, Cel. Antonio Rodrigues Magalhães, 359 Sobral, CE, Brazil.
State University of Acaraú Valley, 62040-370 Sobral, CE, Brazil.
ScientificWorldJournal. 2017;2017:4962707. doi: 10.1155/2017/4962707. Epub 2017 Aug 3.
In vitro antimicrobial and antibiofilm activities of the essential oil and its major components (citral and carvone) against were investigated. Essential oils (LA1EO, LA2EO, and LA3EO) were extracted from the aerial parts of three specimens by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer. Minimum Inhibitory Concentrations (MIC) and Minimum Bacterial Concentration (MBC) were determined by the microdilution method. For the antibiofilm assays, the biomass formation in the biofilm was evaluated by the microtiter-plate technique with the crystal violet (CV) assay and the viability of the bacterial cells was analyzed. All oils and their major components presented antibacterial activity, and the lowest MIC and MBC values were 0.5 mg mL when LA1EO and citral were used. Potential inhibition (100%) of biofilm formation at the concentration of 0.5 mg mL of all EOs was observed. However, the elimination of biofilm cells was confirmed at concentrations of 1 mg mL, 2 mg mL, 2 mg mL, and 0.5 mg mL for LA1EO, LA2EO, LA3EO, and citral, respectively. The results obtained in the present research point to the promising antibacterial and antibiofilm potential of EOs against , a species of recognized clinical interest.
研究了该精油及其主要成分（柠檬醛和香芹酮）对[具体对象未给出]的体外抗菌和抗生物膜活性。通过水蒸馏法从三个[具体植物未给出]标本的地上部分提取精油（LA1EO、LA2EO和LA3EO），并采用气相色谱 - 质谱联用仪进行分析。采用微量稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。对于抗生物膜试验，采用微量滴定板技术和结晶紫（CV）试验评估生物膜中的生物量形成，并分析细菌细胞的活力。所有精油及其主要成分均表现出抗菌活性，当使用LA1EO和柠檬醛时，最低MIC和MBC值为0.5 mg/mL。在所有精油浓度为0.5 mg/mL时观察到对生物膜形成的潜在抑制率为100%。然而，分别在LA1EO、LA2EO、LA3EO和柠檬醛浓度为1 mg/mL、2 mg/mL、2 mg/mL和0.5 mg/mL时证实了生物膜细胞的消除。本研究获得的结果表明，[具体植物未给出]精油对[具体对象未给出]具有有前景的抗菌和抗生物膜潜力，[具体对象未给出]是一种具有公认临床意义的物种。
