Zhang K, Wu K, Liu J, Ge S, Xiao Y, Shang Y, Ning Z
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
College of Veterinary Medicine, Shandong Agricultural University, Shandong, China.
Transbound Emerg Dis. 2017 Aug;64(4):1020-1023. doi: 10.1111/tbed.12659. Epub 2017 May 11.
Atypical porcine pestivirus (APPV) have been detected in swine herds from the USA, Germany, the Netherlands, Spain and most recently in Austria, suggesting a wide geographic distribution of this novel virus. Here, for the first time, we reported APPV infection in swine herds in China. Newborn piglets from two separate swine herds in Guangdong province were found showing typical congenital tremors in July and August 2016. RT-PCR, sequencing and phylogenetic analysis showed APPV infection occurred. Phylogenetic analysis showed that Chinese APPV strains, GD1 and GD2, formed independent branch from the USA, Germany and the Netherlands. Nucleotide identities between members of the APPV ranged between 83.1% and 83.5%, and this showed APPV is highly diverse. It is apparent that this provides the first molecular evidence of APPV infection in swine herds in China.
在美国、德国、荷兰、西班牙以及最近在奥地利的猪群中均检测到了非典型猪瘟病毒（APPV），这表明这种新型病毒具有广泛的地理分布。在此，我们首次报告了中国猪群中的APPV感染情况。2016年7月和8月，在广东省两个不同猪群中的新生仔猪被发现出现典型的先天性震颤。逆转录聚合酶链反应（RT-PCR）、测序和系统发育分析表明发生了APPV感染。系统发育分析显示，中国的APPV毒株GD1和GD2与美国、德国和荷兰的毒株形成了独立分支。APPV各成员之间的核苷酸同一性在83.1%至83.5%之间，这表明APPV具有高度多样性。显然，这提供了中国猪群中APPV感染的首个分子证据。
