Division of Cardiology, Department of Internal Medicine, Inje University College of Medicine, Seoul Paik Hospital , 9 Mareunnae-ro, Jung-gu, Seoul 04551, Republic of Korea.
ACS Appl Mater Interfaces. 2017 May 24;9(20):16803-16812. doi: 10.1021/acsami.7b01555. Epub 2017 May 12.
Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1 MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1 MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1 MPCs.
纳米级表面图案化是一种新兴的潜在方法，可以指导干细胞的命运。我们采用了具有三个直径梯度的纳米级柱形梯度图案化细胞培养板[280-360nm（GP 280/360）、200-280nm（GP 200/280）和 120-200nm（GP 120/200）]，并研究了它们对多能胚胎肝激酶 1 阳性中胚层前体细胞（Flk1 MPCs）的命运修饰作用，这些细胞源自胚胎干细胞。我们观察到 Flk1 MPCs 在纳米图案化板上的细胞增殖和集落形成增加。有趣的是，200-280nm 大小的（GP 200/280）柱形表面显著增加了心肌细胞分化和早期心脏标记基因 Mesp1 的表达。梯度纳米图案表面诱导的心肌细胞具有成熟的心脏基因表达的心肌肌节。我们观察到在此过程中 Vinculin 和 p-Cofilin 介导的细胞骨架重组。总之，具有 200-280nm 大小的柱形的梯度纳米图案表面增强了 Flk1 MPCs 中的心肌细胞分化。
