Noriega Ximena, Pérez Francisco J
a Universidad de Chile, Facultad de Ciencias, Depto. Ciencias Ecológicas, Laboratorio de Bioquímica Vegetal, Casilla , Santiago , Chile.
Plant Signal Behav. 2017 Oct 3;12(10):e1321189. doi: 10.1080/15592324.2017.1321189. Epub 2017 May 12.
Single-bud cuttings of Vitis vinifera L exposed to forced growing conditions were used to investigate the involvement of phytohormones, abscisic acid (ABA), auxin (Aux) and cytokinin (CK) in the release of buds from the ED and in bud-sprouting. This artificial system imitates and hastens the natural sprouting that occurs in spring. Temporal expression analysis of genes related to phytohormones synthesis, showed an early drop in the expression of ABA biosynthesis gene that preceded an increase in Aux and CK biosynthesis genes. Bud-break is headed by the activation of all structures of the latent bud, especially the differentiation of the inflorescence and the development of the early stages of floral organs. Therefore, resumption of cell division and increases in respiration are essential for the activation of the bud. Temporal expression analysis of the cell cycle and respiration genes indicate that an increase in cell division go before the increase in respiration. These results, together with results indicating that the cell cycle genes are upregulated by Aux and CK, suggest that the events before the bud-break, start with a reduction in ABA content, followed by an increase in the content of Aux and CK, which activates the machinery of the cell cycle, which eventually would cause an increase in respiration.
将欧洲葡萄（Vitis vinifera L）的单芽插条置于强制生长条件下，以研究植物激素脱落酸（ABA）、生长素（Aux）和细胞分裂素（CK）在芽从休眠状态释放以及芽萌发过程中的作用。这个人工系统模拟并加速了春季发生的自然萌发过程。对与植物激素合成相关基因的时序表达分析表明，ABA生物合成基因的表达先出现早期下降，随后Aux和CK生物合成基因的表达增加。芽的萌发首先是潜伏芽所有结构的激活，特别是花序的分化和花器官早期阶段的发育。因此，细胞分裂的恢复和呼吸作用的增强对于芽的激活至关重要。对细胞周期和呼吸作用基因的时序表达分析表明，细胞分裂的增加先于呼吸作用的增加。这些结果，连同表明细胞周期基因被Aux和CK上调的结果，表明在芽萌发之前的事件始于ABA含量的降低，随后Aux和CK含量增加，这激活了细胞周期机制，最终导致呼吸作用增强。
