Gurish M F, Ben-Porat T, Nisonoff A
Department of Biology, Rosenstiel Research Center, Brandeis University, Waltham, MA 02254.
Ann Inst Pasteur Immunol. 1988 Nov-Dec;139(6):677-87. doi: 10.1016/0769-2625(88)90056-6.
We have induced the expression of high titres of antibodies to pseudorabies virus (PRV) by immunization of A/J mice with polyclonal antiidiotypic antibodies directed against each of 2 BALB/c monoclonal, neutralizing anti-PRV antibodies. The anti-PRV antibodies induced by one of the antiidiotypic antibody preparations had an affinity for PRV comparable to that of the original monoclonal antibody. Mice immunized with antiidiotypic antibodies were protected to a significant degree against viral infection but the degree of protection was lower than that conferred by passive transfer of monoclonal neutralizing antibody or by immunization with attenuated virus.
我们通过用针对2种BALB/c单克隆中和抗伪狂犬病病毒（PRV）抗体的多克隆抗独特型抗体免疫A/J小鼠，诱导产生了高滴度的抗PRV抗体。其中一种抗独特型抗体制备物诱导产生的抗PRV抗体对PRV的亲和力与原始单克隆抗体相当。用抗独特型抗体免疫的小鼠在很大程度上受到了病毒感染的保护，但保护程度低于单克隆中和抗体的被动转移或减毒病毒免疫所提供的保护程度。
