针对仙台病毒的单克隆抗体。II. 单克隆抗独特型抗体的产生。

Monoclonal antibodies specific for Sendai virus. II. Production of monoclonal anti-idiotypic antibodies.

作者信息

Fenner M, Siegmann K, Binz H

出版信息

Scand J Immunol. 1986 Sep;24(3):341-9. doi: 10.1111/j.1365-3083.1986.tb02103.x.

DOI:10.1111/j.1365-3083.1986.tb02103.x

Abstract

The monoclonal antibodies A and B specific for the HN molecule of Sendai virus were used to induce polyclonal and monoclonal anti-idiotypic antibodies. No response was observed in allogeneic Lewis rats, low responses in syngeneic LOU rats, and high responses in allogeneic BN rats and xenogeneic Balb/c mice. The monoclonals A and B share a similar or identical idiotype, since polyclonal anti-idiotypic antisera to antibody A cross-reacted completely with antibody B and vice versa. The same was found with the three monoclonal anti-idiotypes 1, 2, and 3 elicited in a BN rat or in Balb/c mice. None of the polyclonal or monoclonal anti-idiotypes reacted with other monoclonal antibodies specific for Sendai virus, even when these recognized the same epitope as antibodies A and B. The monoclonal anti-idiotypic antibodies could be used to induce anti-Sendai antibodies in BN rats.

摘要

使用针对仙台病毒HN分子的单克隆抗体A和B诱导多克隆和单克隆抗独特型抗体。在同种异体Lewis大鼠中未观察到反应，在同基因LOU大鼠中反应较弱，而在同种异体BN大鼠和异种Balb/c小鼠中反应较强。单克隆抗体A和B具有相似或相同的独特型，因为针对抗体A的多克隆抗独特型抗血清与抗体B完全交叉反应，反之亦然。在BN大鼠或Balb/c小鼠中诱导产生的三种单克隆抗独特型抗体1、2和3也发现了同样的情况。多克隆或单克隆抗独特型抗体均未与其他针对仙台病毒的单克隆抗体发生反应，即使这些抗体识别与抗体A和B相同的表位。单克隆抗独特型抗体可用于在BN大鼠中诱导抗仙台病毒抗体。