弗氏佐剂与TiterMax在诱导针对伪狂犬病病毒抗原的抗独特型至独特型抗体方面的比较。

Comparison of Freund's adjuvant and TiterMax in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens.

作者信息

Zhou E M, Afshar A

机构信息

Virology Section, Animal Diseases Research Institute, Nepean, Ont., Canada.

出版信息

Vet Immunol Immunopathol. 1995 Sep;48(1-2):113-22. doi: 10.1016/0165-2427(94)05418-r.

DOI:10.1016/0165-2427(94)05418-r

PMID:8533307

Abstract

Freund's adjuvant (FA) and TiterMax (TM) were compared for their effectiveness in the induction of the anti-idiotypic antibodies (anti-Id or Ab2) in pigs, goats and mice against swine polyclonal anti-pseudorabies virus (PRV) antibodies (Ab1) by sequential immunization procedures. Both adjuvants had similar effects on inducing anti-swine immunoglobulin antibodies in the animals. However, high levels of the anti-Id were only generated in animals that received FA. Serological characterization of the anti-Id antisera indicated that internal image anti-Id (Ab2 beta) was generated that recognized shared idiotype (IdX) on the antibodies to PRV. The internal image Ab2 was capable of blocking the anti-PRV antibodies from binding to the PRV antigens, and their interaction with anti-PRV antibodies could be inhibited by PRV antigens.

摘要

通过序贯免疫程序，比较了弗氏佐剂（FA）和泰乐菌素（TM）在猪、山羊和小鼠中诱导抗独特型抗体（抗Id或Ab2）以对抗猪多克隆抗伪狂犬病病毒（PRV）抗体（Ab1）的效果。两种佐剂在诱导动物产生抗猪免疫球蛋白抗体方面具有相似的作用。然而，只有接受FA的动物产生了高水平的抗Id。抗Id抗血清的血清学特征表明，产生了识别PRV抗体上共享独特型（IdX）的内影像抗Id（Ab2β）。内影像Ab2能够阻断抗PRV抗体与PRV抗原的结合，并且它们与抗PRV抗体的相互作用可被PRV抗原抑制。