Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun 130012, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun 130012, China.
Talanta. 2017 Aug 1;170:286-290. doi: 10.1016/j.talanta.2017.03.081. Epub 2017 Mar 27.
In this work, we have designed a novel kind of nanohybrid with magnetic and photoluminescence (PL) property for trypsin detection. The modified magnetic FeO nanoparticles (MNPs) with polydopamine (PDA) and human serum albumin (HSA) were prepared through a one step self-polymerization under mild condition. The polydopamine (PDA) coating on MNPs can improve the biocompatibility of the MNP-PDA-HAS composite due to its hydrophilicity and multifunctional groups. When MNP-PDA-HSA composite was added into the Anti-HSA modified CdTe QDs (anti-HSA-QDs), HSA on the MNP-PDA-HSA composite was captured by the site of anti-HSA-QDs to form MNP-PDA-HSA/anti-HSA-QDs nanohybrid. Therefore, the photoluminescence of QDs can be quenched by FeO nanoparticles due to the electron transfer. In the presence of trypsin, the protein (anti-HSA) was digested by trypsin and QDs was separated from the nanohybrid surface. As a result, the photoluminescence intensity of QDs was recovered. The magnetic/luminescent bifunctional nanohybrid displayed excellent analytical performance for the detection of trypsin in the range of 0.5-30μg/mL with a low detection limit of 0.25μg/mL.
在这项工作中，我们设计了一种具有磁性和光致发光（PL）性能的新型纳米杂化材料，用于胰蛋白酶检测。通过在温和条件下的一步自聚合，制备了修饰有聚多巴胺（PDA）和人血清白蛋白（HSA）的改性磁性 FeO 纳米颗粒（MNPs）。MNPs 上的聚多巴胺（PDA）涂层由于其亲水性和多功能基团，可以提高 MNP-PDA-HAS 复合材料的生物相容性。当 MNP-PDA-HSA 复合材料添加到抗-HSA 修饰的 CdTe QDs（抗-HSA-QDs）中时，MNP-PDA-HSA 复合材料上的 HSA 被抗-HSA-QDs 的位点捕获，形成 MNP-PDA-HSA/抗-HSA-QDs 纳米杂化物。因此，由于电子转移，FeO 纳米颗粒可以猝灭 QDs 的光致发光。在存在胰蛋白酶的情况下，蛋白质（抗-HSA）被胰蛋白酶消化，QDs 从纳米杂化物表面分离。结果，QDs 的光致发光强度得到恢复。这种磁性/发光双功能纳米杂化物在 0.5-30μg/mL 的范围内对胰蛋白酶的检测表现出优异的分析性能，检测限低至 0.25μg/mL。
