基于 CdTe QDs/nano-FeO@MIPs 磁性荧光探针检测鱼组织中的孔雀石绿。
Detection of malachite green in fish based on magnetic fluorescent probe of CdTe QDs/nano-FeO@MIPs.
机构信息
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5;196:117-122. doi: 10.1016/j.saa.2018.02.016. Epub 2018 Feb 7.
A magnetic fluorescent probe of CdTe QDs/nano-FeO@MIPs was prepared using CdTe QDs and FeO nanoparticles as co-nucleus and molecularly imprinted polymers (MIPs) as specific recognition sites based on a reverse microemulsion method. With the specific enrichment and magnetic separation properties, the probe of CdTe QDs/nano-FeO@MIPs was used to detect malachite green (MG) in fish samples. The TEM analysis showed that the particles of CdTe QDs/nano-FeO@MIPs were spherical with average diameter around 53nm, and a core-shell structure was well-shaped with several FeO nanoparticles and CdTe QDs embedded in each of the microsphere. Quick separation of the probes from solutions could be realized with a magnet, indicating the excellent magnetic property of CdTe QDs/nano-FeO@MIPs. The probe exhibited high specific adsorption towards MG and excellent fluorescence emission at λ 598nm. The fluorescence of CdTe QDs/nano-FeO@MIPs could be linearly quenched by MG at the concentrations from 0.025 to 1.5μmolL. The detection limit was 0.014μmolL. The average recovery of spiked MG in fish samples was 105.2%. The result demonstrated that the as-prepared CdTe QDs/nano-FeO@MIPs could be used as a probe to the detection of trace MG in fish samples.
基于反相微乳液法，以 CdTe QDs 和 FeO 纳米粒子为共核，以分子印迹聚合物（MIPs）为特异性识别位点，制备了 CdTe QDs/nano-FeO@MIPs 磁性荧光探针。该探针具有特异性富集和磁分离性能，用于检测鱼样中的孔雀石绿（MG）。TEM 分析表明，CdTe QDs/nano-FeO@MIPs 颗粒呈球形，平均直径约为 53nm，形成了良好的核壳结构，每个微球中嵌入了几个 FeO 纳米粒子和 CdTe QDs。通过磁铁可以快速将探针从溶液中分离出来，表明 CdTe QDs/nano-FeO@MIPs 具有优异的磁性。该探针对 MG 表现出高特异性吸附和出色的荧光发射，在 λ 598nm 处。CdTe QDs/nano-FeO@MIPs 的荧光可以被 MG 在 0.025 至 1.5μmolL 的浓度范围内线性猝灭。检测限为 0.014μmolL。鱼样中 MG 的平均回收率为 105.2%。结果表明，所制备的 CdTe QDs/nano-FeO@MIPs 可作为探针用于检测鱼样中的痕量 MG。
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