Yüzüncü Yıl University, Faculty of Science, Department of Analytical Chemistry, 65080 Van, Turkey.
Yüzüncü Yıl University, Faculty of Pharmacy, Department of Analytical Chemistry, 65080 Van, Turkey.
Talanta. 2017 Aug 1;170:384-391. doi: 10.1016/j.talanta.2017.04.037. Epub 2017 Apr 17.
This paper describes an electroanalytical method for the simultaneous determination of vanillin (VAN) and caffeine (CAF) using an anodically pretreated boron-doped diamond electrode. Selective determination of one compound in the presence of other one was also realized. Both compounds yielded a single irreversible oxidation peak using cyclic voltammetry. The nature of the electrode reaction was found to be diffusion controlled with contribution of adsorption. By using square-wave adsorptive stripping voltammetry after 60s accumulation under open-circuit voltage, method allowed simultaneous determination of VAN and CAF in phosphate buffer, pH 2.5, with detection limits of 0.234µgmL (1.54×10M) and 0.071µgmL (3.66×10M), respectively. The proposed method was successfully applied in the selective and simultaneous determination of VAN and CAF in commercial food and beverage samples. In addition, for the comparison, high-performance liquid chromatographic method with diode-array detection was developed for the first time for their simultaneous determination.
本文描述了一种使用阳极预处理的掺硼金刚石电极同时测定香草醛（VAN）和咖啡因（CAF）的电化学分析方法。还实现了在存在一种化合物的情况下对另一种化合物的选择性测定。两种化合物在循环伏安法中都产生了一个单一的不可逆氧化峰。电极反应的性质被发现是扩散控制的，同时存在吸附作用的贡献。通过在开路电压下 60s 累积后使用方波吸附溶出伏安法，可以在 pH 2.5 的磷酸盐缓冲液中同时测定 VAN 和 CAF，检测限分别为 0.234µgmL（1.54×10M）和 0.071µgmL（3.66×10M）。该方法成功地应用于商业食品和饮料样品中 VAN 和 CAF 的选择性和同时测定。此外，为了进行比较，首次开发了带有二极管阵列检测的高效液相色谱法用于它们的同时测定。
