Van Yuzuncu Yil University, Faculty of Science, Department of Biochemistry, 65080, Van, Turkey.
Van Yuzuncu Yil University, Faculty of Medicine, Department of Pharmacology, 65080, Van, Turkey.
Anal Chim Acta. 2021 May 15;1159:338418. doi: 10.1016/j.aca.2021.338418. Epub 2021 Mar 18.
Favipiravir, a promising antiviral agent, is undergoing clinical trials for the potential treatment of the novel coronavirus disease 2019 (COVID-19). This is the first report for the electrochemical activity of favipiravir and its electroanalytical sensing. For this purpose, the effect of cationic surfactant, CTAB was demonstrated on the enhanced accumulation of favipiravir at the surface of cathodically pretreated boron-doped diamond (CPT-BDD) electrode. At first, the electrochemical properties of favipiravir were investigated in the surfactant-free solutions by the means of cyclic voltammetry. The compound presented a single oxidation step which is irreversible and adsorption controlled. A systematic study of various operational conditions, such as electrode pretreatment, pH of the supporting electrolyte, concentration of CTAB, accumulation variables, and instrumental parameters on the adsorptive stripping response, was examined using square-wave voltammetry. An oxidation signal at around +1.21 V in Britton-Robinson buffer at pH 8.0 containing 6 × 10 M CTAB allowed to the adsorptive stripping voltammetric determination of favipiravir (after 60 s accumulation step at open-circuit condition). The process could be used in the concentration range with two linear segments of 0.01-0.1 μg mL (6.4 × 10-6.4 × 10 M) and 0.1-20.0 μg mL (6.4 × 10-1.3 × 10 M). The limit of detection values were found to be 0.0028 μg mL (1.8 × 10 M), and 0.023 μg mL (1.5 × 10 M) for the first and second segments of calibration graph, respectively. The feasibility of developed methodology was tested to the analysis of the commercial tablet formulations and model human urine samples.
法匹拉韦是一种有前途的抗病毒药物，目前正在进行临床试验，以探索其在治疗 2019 年新型冠状病毒病（COVID-19）方面的潜力。这是首次报道法匹拉韦的电化学活性及其电化学分析传感。为此，研究了阳离子表面活性剂 CTAB 对在阴极预处理的掺硼金刚石（CPT-BDD）电极表面富集法匹拉韦的增强作用。首先，在无表面活性剂的溶液中，通过循环伏安法研究了法匹拉韦的电化学性质。该化合物呈现出单一的不可逆吸附控制的氧化步骤。通过使用方波伏安法，系统地研究了各种操作条件，如电极预处理、支持电解质的 pH 值、CTAB 浓度、富集变量和仪器参数对吸附溶出响应的影响。在 Britton-Robinson 缓冲液（pH 8.0 中含有 6×10-6M CTAB）中，在+1.21V 左右可得到一个氧化信号，允许在开路条件下进行 60s 的吸附溶出伏安法测定法匹拉韦（在+1.21V 左右可得到一个氧化信号，允许在开路条件下进行 60s 的吸附溶出伏安法测定法匹拉韦）。该过程可用于 0.01-0.1μgmL（6.4×10-6.4×10-6M）和 0.1-20.0μgmL（6.4×10-1.3×10-6M）的浓度范围内。检测限分别为 0.0028μgmL（1.8×10-6M）和 0.023μgmL（1.5×10-6M）。该方法已成功应用于商业片剂制剂和模型人尿样的分析。
