Bcg基因的多效性作用：III. Bcg同基因巨噬细胞中的呼吸爆发
Pleiotropic effects of the Bcg gene: III. Respiratory burst in Bcg-congenic macrophages.
作者信息
Denis M, Forget A, Pelletier M, Skamene E
机构信息
Département de Microbiologie et d'Immunologie, Université de Montréal, Québec.
出版信息
Clin Exp Immunol. 1988 Sep;73(3):370-5.
Macrophage respiratory burst, as assessed by H2O2 and O2- production, and HMS and chemiluminescence activity was investigated in a variety of conditions in macrophages from Bcg-congenic mice. Measurement of HMS and chemiluminescence in splenic macrophages challenged in vitro with BCG showed that the Bcgr cells were more stimulated by the challenge than their Bcgs counterparts. H2O2 production was measured in Bcgr and Bcgs splenic macrophages. PMA-triggering and LK-triggering were shown to stimulate a similar degree of H2O2 production Bcgr and Bcgs macrophages. In contrast, in vitro phagocytosis of BCG was shown to trigger superior production of H2O2 and O2- in the Bcgr splenic macrophages as compared to their Bcgs congenics. Finally, following the in vivo infection with BCG Montreal, Bcgr splenic macrophages were superior producers of H2O2 (both spontaneous and PMA-triggered) in the early phase of infection.
通过H2O2和O2-生成以及己糖单磷酸支路（HMS）和化学发光活性评估的巨噬细胞呼吸爆发，在来自卡介苗（Bcg）同源小鼠的巨噬细胞的各种条件下进行了研究。对体外受到卡介苗攻击的脾巨噬细胞中的HMS和化学发光进行测量，结果显示Bcgr细胞比其Bcgs对应细胞受到攻击的刺激更大。在Bcgr和Bcgs脾巨噬细胞中测量了H2O2的生成。已表明佛波醇肉豆蔻酸酯（PMA）触发和脂多糖（LK）触发对Bcgr和Bcgs巨噬细胞刺激的H2O2生成程度相似。相比之下，与它们的Bcgs同源细胞相比，体外吞噬卡介苗显示在Bcgr脾巨噬细胞中触发了更高水平的H2O2和O2-生成。最后，在用蒙特利尔卡介苗进行体内感染后，在感染早期，Bcgr脾巨噬细胞是H2O2（自发和PMA触发）的优势产生者。
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