Department of Fundamental and Applied Sciences, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia.
Department of Fundamental and Applied Sciences, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia.
Bioresour Technol. 2017 Sep;239:127-136. doi: 10.1016/j.biortech.2017.04.118. Epub 2017 May 4.
The potential to grow attached microalgae Chlorella vulgaris in fluidized bed bioreactor was materialized in this study, targeting to ease the harvesting process prior to biodiesel production. The proposed thermodynamic mechanism and physical property assessment of various support materials verified polyurethane to be suitable material favouring the spontaneous adhesion by microalgae cells. The 1-L bioreactor packed with only 2.4% (v/v) of 1.00-mL polyurethane foam cubes could achieve the highest attached growth microalgae biomass and lipid weights of 812±122 and 376±37mg, respectively, in comparison with other cube sizes. The maturity of attached growth microalgae biomass for harvesting could also be determined from the growth trend of suspended microalgae biomass. Analysis of FAME composition revealed that the harvested microalgae biomass was dominated by C16-C18 (>60%) and mixture of saturated and mono-unsaturated fatty acids (>65%), satiating the biodiesel standard with adequate cold flow property and oxidative stability.
本研究实现了在流化床生物反应器中培养附着微藻小球藻的潜力，旨在简化生物柴油生产前的收获过程。对各种支撑材料的热力学机制和物理性能评估表明，聚氨酯是一种合适的材料，有利于微藻细胞的自发附着。在 1 升生物反应器中，仅填充 2.4%（v/v）的 1.00 毫升聚氨酯泡沫块，可获得最高的附着生长微藻生物量和脂质重量，分别为 812±122 和 376±37mg，与其他块大小相比。从悬浮微藻生物量的生长趋势也可以确定附着生长微藻生物量的成熟度，以进行收获。脂肪酸甲酯（FAME）组成分析表明，收获的微藻生物质主要由 C16-C18（>60%）和饱和和单不饱和脂肪酸的混合物（>65%）组成，具有足够的冷流性能和氧化稳定性，满足生物柴油标准。
