Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, 050011, China.
College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, 050024, China.
J Fluoresc. 2017 Sep;27(5):1739-1745. doi: 10.1007/s10895-017-2112-4. Epub 2017 May 13.
Based on the photoinduced electron transfer (PET) principle, 4-amino-7-nitro-2,1,3-benzoxadiazole (ANBD) has been used as a fluorophore to develop a new fluorescent probe, 4-(2-N,N-dimethylthioacetamide)amino-7-nitro-2,1,3-benzoxadiazole (2), for the detection of Hg. Upon the addition of Hg, a 46-fold fluorescence enhancement occurs. Moreover the probe 2 exhibits a high selectivity and sensitivity to Hg, even in the presence of other common metal ions. Under optimal reaction conditions, a good linearity can be obtained in the range of 0.5-2.5 μM, and the detection limit is 0.05 μM. In addition, the desulfurization reaction mechanism is proposed based on electrospray ionization mass spectrum. The present study is not only a supplement to the detection method of Hg, but also a merit to the development of ANBD-based fluorescent probes.
基于光诱导电子转移（PET）原理，4-氨基-7-硝基-2,1,3-苯并恶二唑（ANBD）被用作荧光团，开发了一种新的荧光探针 4-(2-N,N-二甲基硫代乙酰胺)氨基-7-硝基-2,1,3-苯并恶二唑（2），用于检测 Hg。加入 Hg 后，荧光增强了 46 倍。此外，探针 2 对 Hg 具有高选择性和灵敏度，即使存在其他常见金属离子也是如此。在最佳反应条件下，在 0.5-2.5 μM 范围内可以得到良好的线性关系，检测限为 0.05 μM。此外，还基于电喷雾电离质谱提出了脱硫反应机理。本研究不仅是 Hg 检测方法的补充，也是基于 ANBD 的荧光探针发展的一个优点。
