Affiliated Hospital of Shandong Academy of Medical Sciences, Shandong First Medical University, Jinan 250031, Shandong, China.
Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China.
Bioorg Med Chem Lett. 2020 Jul 1;30(13):127221. doi: 10.1016/j.bmcl.2020.127221. Epub 2020 Apr 27.
A new Methylene blue-based 7-nitro-1,2,3-benzoxadiazole NIR fluorescent probe 3, 7-bis-dimethylamino-10-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)-10H-phenothiazine (leuco-MB-NBD) was designed and synthesized. Leuco-MB-NBD showed high sensitivity and selectivity for HS as a fluorescent probe in CHOH-PBS (9:1, v/v, pH = 7.4) solution, this fluorescent assay showed a linear range of 0-50.0 μM and a LOD (limit of detection) of 0.43 μM. Moreover, the probe leuco-MB-NBD has lower toxicity at low concentrations to HCT-116 cells and can be used for cell imaging. Additionally, Leuco-MB-NBD is triggered by hydrogen sulfide to generate methylene blue, methylene blue which has potential rescuing effects on the mitochondrial activity can act as an antidote against sulfide intoxication.
一种新型的亚甲蓝基 7-硝基-1,2,3-苯并恶二唑近红外荧光探针 3,7-双二甲氨基-10-(7-硝基苯并[c][1,2,5]恶二唑-4-基)-10H-吩噻嗪（亮甲亚蓝-NBD）被设计和合成。亮甲亚蓝-NBD 作为一种荧光探针，在 CHOH-PBS（9:1，v/v，pH=7.4）溶液中对 HS 表现出高灵敏度和选择性，这种荧光测定法显示出 0-50.0 μM 的线性范围和 0.43 μM 的检测限（LOD）。此外，探针亮甲亚蓝-NBD 在低浓度时对 HCT-116 细胞的毒性较低，可用于细胞成像。此外，亮甲亚蓝-NBD 被硫化氢触发生成亚甲蓝，亚甲蓝对线粒体活性具有潜在的挽救作用，可以作为对抗硫化物中毒的解毒剂。
