Nguyen Jenny Ngoc Tran, Harbison Amanda M
Northern Virginia Community College, Manassas Campus, 6901 Sudley Road, Manassas, VA, 20109, USA.
Methods Mol Biol. 2017;1606:71-84. doi: 10.1007/978-1-4939-6990-6_5.
Scanning electron microscopes allow us to reach magnifications of 20-130,000× and resolve compositional and topographical images with intense detail. These images are created by bombarding a sample with electrons in a focused manner to generate a black and white image from the electrons that bounce off of the sample. The electrons are detected using positively charged detectors. Scanning electron microscopy permits three-dimensional imaging of desiccated specimens or wet cells and tissues by using variable pressure chambers. SEM ultrastructural analysis and intracellular imaging supplement light microscopy for molecular profiling of prokaryotes, plants, and mammals. This chapter demonstrates how to prepare and image samples that are (a) desiccated and conductive, (b) desiccated and nonconductive but coated with an electron conductive film using a gold sputter coater, and
扫描电子显微镜使我们能够达到20至130,000倍的放大倍数，并以极高的细节解析成分和形貌图像。这些图像是通过将电子聚焦轰击样品，使从样品反弹的电子生成黑白图像而形成的。使用带正电荷的探测器来检测电子。扫描电子显微镜通过使用可变压力室，允许对干燥标本或湿细胞及组织进行三维成像。扫描电子显微镜超微结构分析和细胞内成像补充了光学显微镜，用于原核生物、植物和哺乳动物的分子分析。本章展示了如何制备和成像以下样品：（a）干燥且导电的样品；（b）干燥且不导电但使用金溅射镀膜机涂覆有电子导电膜的样品，以及
