脱细胞颈动脉支架及其组织工程生物学和生物力学性能评估
Acellular carotid scaffold and evaluation the biological and biomechanical properties for tissue engineering.
作者信息
Rashidi Farina, Mohammadzadeh Mehdi, Abdolmaleki Arash, Asadi Asadollah, Sheikhlou Mehrdad
机构信息
Department of Biology, Faculty of Science, University of Urmia, Urmia, Iran.
Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran.
出版信息
J Cardiovasc Thorac Res. 2024;16(1):28-37. doi: 10.34172/jcvtr.32899. Epub 2024 Mar 13.
INTRODUCTION
The issues associated with the limitation of appropriate autologous vessels for vascular reconstruction via bypass surgery highlight the need for new alternative strategies based on tissue engineering. The present study aimed to prepare decellularized scaffolds from ovine carotid using chemical decellularization method.
METHODS
Ovine carotid were decellularized with Triton X-100 and tri-n-butyl phosphate (TnBP) at 37 °C. Histological analysis, biochemical tests, biomechanical assay and biocompatibility assay were used to investigate the efficacy of decellularization.
RESULTS
Decellularization method could successfully decellularize ovine carotid without leaving any cell remnants. Scaffolds showed minimal destruction of the three-dimensional structure and extracellular matrix, as well as adequate mechanical resistance and biocompatibility for cell growth and proliferation.
CONCLUSION
Prepared acellular scaffold exhibited the necessary characteristics for clinical applications.
引言
通过旁路手术进行血管重建时，合适的自体血管存在局限性，这凸显了基于组织工程的新替代策略的必要性。本研究旨在使用化学去细胞化方法从绵羊颈动脉制备去细胞化支架。
方法
在37°C下，用 Triton X - 100和磷酸三丁酯（TnBP）对绵羊颈动脉进行去细胞化处理。采用组织学分析、生化测试、生物力学测定和生物相容性测定来研究去细胞化的效果。
结果
去细胞化方法能够成功去除绵羊颈动脉的细胞，且不留任何细胞残余物。支架的三维结构和细胞外基质破坏最小，对细胞生长和增殖具有足够的机械抗性和生物相容性。
结论
制备的无细胞支架展现出临床应用所需的特性。
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