血管活性肠肽与非肾上腺素能非胆碱能神经元刺激对猫胃底作用的比较。
Comparison of the effect of vasoactive intestinal polypeptide and non-adrenergic non-cholinergic neurone stimulation in the cat gastric fundus.
作者信息
D'Amato M, De Beurme F A, Lefebvre R A
机构信息
Heymans Institute of Pharmacology, University of Gent Medical School, Belgium.
出版信息
Eur J Pharmacol. 1988 Jul 26;152(1-2):71-82. doi: 10.1016/0014-2999(88)90837-0.
The possible involvement of vasoactive intestinal polypeptide (VIP) in the non-adrenergic non-cholinergic (NANC) relaxation of the cat gastric fundus was studied in circular and longitudinal muscle strips. Cumulative transmural stimulation induced a frequency-dependent relaxation, while cumulative administration of VIP induced a concentration-dependent relaxation. Tetrodotoxin almost completely antagonized the relaxation induced by transmural stimulation (1 Hz), but did not influence the relaxation induced by VIP (10(-7) M); the latter was not influenced by hexamethonium or propranolol plus phentolamine. Trypsin (30 min incubation) and VIP antiserum (1 h incubation) prevented the relaxation induced by VIP and reduced that induced by transmural stimulation, but did not influence the relaxation induced by isopropylnoradrenaline. Two putative VIP receptor antagonists, [AcTyr1]hGRF-(1-40)OH and [4Cl-D-Phe6,Leu17]VIP, did not influence the relaxation induced by VIP or transmural stimulation. These results are compatible with the hypothesis that VIP is involved in the NANC relaxation of the cat gastric fundus, although participation of a non-VIP component cannot be excluded.
在猫胃底的环形和纵形肌条中，研究了血管活性肠肽（VIP）参与非肾上腺素能非胆碱能（NANC）舒张的可能性。累积经壁刺激可诱导频率依赖性舒张，而累积给予VIP可诱导浓度依赖性舒张。河豚毒素几乎完全拮抗经壁刺激（1Hz）诱导的舒张，但不影响VIP（10⁻⁷M）诱导的舒张；后者不受六甲铵或普萘洛尔加酚妥拉明的影响。胰蛋白酶（孵育30分钟）和VIP抗血清（孵育1小时）可阻止VIP诱导的舒张，并减弱经壁刺激诱导的舒张，但不影响异丙去甲肾上腺素诱导的舒张。两种假定的VIP受体拮抗剂，[AcTyr1]hGRF-(1-40)OH和[4Cl-D-Phe6,Leu17]VIP，不影响VIP或经壁刺激诱导的舒张。这些结果与VIP参与猫胃底NANC舒张的假说相符，尽管不能排除非VIP成分的参与。
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