α-糜蛋白酶和胰蛋白酶对大鼠胃底非肾上腺素能非胆碱能舒张的影响。
Influence of alpha-chymotrypsin and trypsin on the non-adrenergic non-cholinergic relaxation in the rat gastric fundus.
作者信息
De Beurme F A, Lefebvre R A
出版信息
Br J Pharmacol. 1987 May;91(1):171-7. doi: 10.1111/j.1476-5381.1987.tb08996.x.
Abstract
Relaxations of the rat gastric fundus were induced by electrical stimulation of the non-adrenergic non-cholinergic (NANC) neurones, by vasoactive intestinal polypeptide (VIP), by noradrenaline and by isopropylnoradrenaline. The influence of alpha-chymotrypsin and trypsin thereupon was studied. alpha-Chymotrypsin 2 u ml-1, present for 30 min, antagonized completely the VIP-induced relaxation, but not the stimulation-induced relaxation; alpha-chymotrypsin 10 u ml-1 also partially antagonized the stimulation-induced relaxation. When alpha-chymotrypsin 2 u ml-1 was added after the relaxation had occurred, it antagonized completely the VIP-induced relaxation, but it also partially antagonized the stimulation-induced relaxation. The partial antagonism of the stimulation-induced relaxation was more pronounced with alpha-chymotrypsin 10 u ml-1. Trypsin, 10(-6) M and 3 X 10(-6) M, had effects on VIP- and stimulation-induced relaxations similar to those of alpha-chymotrypsin. The relaxations induced by noradrenaline and isopropylnoradrenaline were not influenced by alpha-chymotrypsin or trypsin, respectively. The results suggest that a peptide, possibly VIP, is involved in the NANC relaxation of the rat gastric fundus.
摘要
通过电刺激非肾上腺素能非胆碱能（NANC）神经元、血管活性肠肽（VIP）、去甲肾上腺素和异丙肾上腺素可诱导大鼠胃底松弛。研究了α-糜蛋白酶和胰蛋白酶对其的影响。2 U/ml的α-糜蛋白酶作用30分钟，可完全拮抗VIP诱导的松弛，但不能拮抗电刺激诱导的松弛；10 U/ml的α-糜蛋白酶也可部分拮抗电刺激诱导的松弛。当在松弛发生后加入2 U/ml的α-糜蛋白酶时，它可完全拮抗VIP诱导的松弛，但也可部分拮抗电刺激诱导的松弛。10 U/ml的α-糜蛋白酶对电刺激诱导的松弛的部分拮抗作用更明显。10⁻⁶ M和3×10⁻⁶ M的胰蛋白酶对VIP和电刺激诱导的松弛的作用与α-糜蛋白酶相似。去甲肾上腺素和异丙肾上腺素诱导的松弛分别不受α-糜蛋白酶或胰蛋白酶的影响。结果表明，一种肽，可能是VIP，参与了大鼠胃底的NANC松弛。
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