α-糜蛋白酶和胰蛋白酶对大鼠胃底非肾上腺素能非胆碱能舒张的影响。
Influence of alpha-chymotrypsin and trypsin on the non-adrenergic non-cholinergic relaxation in the rat gastric fundus.
作者信息
De Beurme F A, Lefebvre R A
出版信息
Br J Pharmacol. 1987 May;91(1):171-7. doi: 10.1111/j.1476-5381.1987.tb08996.x.
Abstract
Relaxations of the rat gastric fundus were induced by electrical stimulation of the non-adrenergic non-cholinergic (NANC) neurones, by vasoactive intestinal polypeptide (VIP), by noradrenaline and by isopropylnoradrenaline. The influence of alpha-chymotrypsin and trypsin thereupon was studied. alpha-Chymotrypsin 2 u ml-1, present for 30 min, antagonized completely the VIP-induced relaxation, but not the stimulation-induced relaxation; alpha-chymotrypsin 10 u ml-1 also partially antagonized the stimulation-induced relaxation. When alpha-chymotrypsin 2 u ml-1 was added after the relaxation had occurred, it antagonized completely the VIP-induced relaxation, but it also partially antagonized the stimulation-induced relaxation. The partial antagonism of the stimulation-induced relaxation was more pronounced with alpha-chymotrypsin 10 u ml-1. Trypsin, 10(-6) M and 3 X 10(-6) M, had effects on VIP- and stimulation-induced relaxations similar to those of alpha-chymotrypsin. The relaxations induced by noradrenaline and isopropylnoradrenaline were not influenced by alpha-chymotrypsin or trypsin, respectively. The results suggest that a peptide, possibly VIP, is involved in the NANC relaxation of the rat gastric fundus.
摘要
通过电刺激非肾上腺素能非胆碱能（NANC）神经元、血管活性肠肽（VIP）、去甲肾上腺素和异丙肾上腺素可诱导大鼠胃底松弛。研究了α-糜蛋白酶和胰蛋白酶对其的影响。2 U/ml的α-糜蛋白酶作用30分钟，可完全拮抗VIP诱导的松弛，但不能拮抗电刺激诱导的松弛；10 U/ml的α-糜蛋白酶也可部分拮抗电刺激诱导的松弛。当在松弛发生后加入2 U/ml的α-糜蛋白酶时，它可完全拮抗VIP诱导的松弛，但也可部分拮抗电刺激诱导的松弛。10 U/ml的α-糜蛋白酶对电刺激诱导的松弛的部分拮抗作用更明显。10⁻⁶ M和3×10⁻⁶ M的胰蛋白酶对VIP和电刺激诱导的松弛的作用与α-糜蛋白酶相似。去甲肾上腺素和异丙肾上腺素诱导的松弛分别不受α-糜蛋白酶或胰蛋白酶的影响。结果表明，一种肽，可能是VIP，参与了大鼠胃底的NANC松弛。
相似文献
1
Influence of alpha-chymotrypsin and trypsin on the non-adrenergic non-cholinergic relaxation in the rat gastric fundus.α-糜蛋白酶和胰蛋白酶对大鼠胃底非肾上腺素能非胆碱能舒张的影响。
Br J Pharmacol. 1987 May;91(1):171-7. doi: 10.1111/j.1476-5381.1987.tb08996.x.
2
Comparison of the effect of vasoactive intestinal polypeptide and non-adrenergic non-cholinergic neurone stimulation in the cat gastric fundus.血管活性肠肽与非肾上腺素能非胆碱能神经元刺激对猫胃底作用的比较。
Eur J Pharmacol. 1988 Jul 26;152(1-2):71-82. doi: 10.1016/0014-2999(88)90837-0.
3
Vasoactive intestinal polypeptide as possible mediator of relaxation in the rat gastric fundus.血管活性肠肽作为大鼠胃底舒张的可能介质。
J Pharm Pharmacol. 1988 Oct;40(10):711-5. doi: 10.1111/j.2042-7158.1988.tb07000.x.
4
Involvement of nitric oxide in non-adrenergic non-cholinergic relaxation and action of vasoactive intestinal polypeptide in circular muscle strips of the rat gastric fundus.一氧化氮参与大鼠胃底环形肌条中非肾上腺素能非胆碱能舒张及血管活性肠肽的作用。
Pharmacol Res. 2001 Sep;44(3):221-8. doi: 10.1006/phrs.2001.0844.
5
Influence of NG-nitro-L-arginine on non-adrenergic non-cholinergic relaxation in the guinea-pig gastric fundus.NG-硝基-L-精氨酸对豚鼠胃底非肾上腺素能非胆碱能舒张的影响。
Br J Pharmacol. 1992 May;106(1):173-9. doi: 10.1111/j.1476-5381.1992.tb14311.x.
6
Modulation of non-adrenergic non-cholinergic inhibitory neurotransmission in rat gastric fundus by the alpha 2-adrenoceptor agonist, UK-14,304.α2-肾上腺素能受体激动剂UK-14,304对大鼠胃底非肾上腺素能非胆碱能抑制性神经传递的调节作用
Br J Pharmacol. 1992 Sep;107(1):256-61. doi: 10.1111/j.1476-5381.1992.tb14495.x.
7
Evidence for dual components in the non-adrenergic non-cholinergic relaxation in the rat gastric fundus: role of endogenous nitric oxide and vasoactive intestinal polypeptide.
J Auton Nerv Syst. 1992 Mar;37(3):175-86. doi: 10.1016/0165-1838(92)90039-j.
8
Effect of diabetes on relaxations to non-adrenergic, non-cholinergic nerve stimulation in longitudinal muscle of the rat gastric fundus.糖尿病对大鼠胃底纵行肌非肾上腺素能、非胆碱能神经刺激所致舒张的影响。
Br J Pharmacol. 1995 Sep;116(1):1551-6. doi: 10.1111/j.1476-5381.1995.tb16372.x.
9
Is peptide histidine isoleucine an inhibitory nonadrenergic noncholinergic neurotransmitter in the rat gastric fundus?肽组氨酸异亮氨酸是大鼠胃底中的一种抑制性非肾上腺素能非胆碱能神经递质吗？
Arch Int Pharmacodyn Ther. 1990 Jan-Feb;303:216-31.
10
Similarities between the relaxations induced by vasoactive intestinal peptide and by stimulation of the non-adrenergic non-cholinergic neurons in the rat stomach.血管活性肠肽诱导的舒张与大鼠胃中非肾上腺素能非胆碱能神经元刺激所诱导的舒张之间的相似性。
Naunyn Schmiedebergs Arch Pharmacol. 1988 Oct;338(4):401-6. doi: 10.1007/BF00172117.
引用本文的文献
1
A possible role of neurotensin in NANC relaxation of longitudinal muscle of the jejunum and ileum of Wistar rats.神经降压素在Wistar大鼠空肠和回肠纵行肌非肾上腺素能非胆碱能舒张中的可能作用。
Br J Pharmacol. 2002 Nov;137(5):629-36. doi: 10.1038/sj.bjp.0704914.
2
Pre- and postjunctional protective effect of neocuproine on the nitrergic neurotransmitter in the mouse gastric fundus.新亚铜试剂对小鼠胃底中氮能神经递质的接头前和接头后保护作用。
Br J Pharmacol. 2001 Jan;132(1):277-85. doi: 10.1038/sj.bjp.0703772.
3
Development of cholinergic and inhibitory non-adrenergic non-cholinergic responses in the rat gastric funds.大鼠胃底胆碱能及抑制性非肾上腺素能非胆碱能反应的发育
Br J Pharmacol. 1996 Aug;118(8):1987-94. doi: 10.1111/j.1476-5381.1996.tb15634.x.
4
ATP and nitric oxide: inhibitory NANC neurotransmitters in the longitudinal muscle-myenteric plexus preparation of the rat ileum.三磷酸腺苷与一氧化氮：大鼠回肠纵行肌-肌间神经丛标本中的抑制性非肾上腺素能非胆碱能神经递质
Br J Pharmacol. 1996 Jun;118(3):695-703. doi: 10.1111/j.1476-5381.1996.tb15456.x.
5
Study of NO and VIP as non-adrenergic non-cholinergic neurotransmitters in the pig gastric fundus.猪胃底中一氧化氮和血管活性肠肽作为非肾上腺素能非胆碱能神经递质的研究。
Br J Pharmacol. 1995 Oct;116(3):2017-26. doi: 10.1111/j.1476-5381.1995.tb16406.x.
6
Peptide histidine isoleucine-like immunoreactivity release from the rat gastric fundus.大鼠胃底中肽组氨酸异亮氨酸样免疫反应性物质的释放
Br J Pharmacol. 1994 Oct;113(2):541-9. doi: 10.1111/j.1476-5381.1994.tb17023.x.
7
5-HT-induced neurogenic relaxations of the guinea-pig proximal colon: investigation into the role of ATP and VIP in addition to nitric oxide.5-羟色胺诱导的豚鼠近端结肠神经源性舒张：除一氧化氮外，对三磷酸腺苷和血管活性肠肽作用的研究
Naunyn Schmiedebergs Arch Pharmacol. 1995 Feb;351(2):126-35. doi: 10.1007/BF00169326.
8
Mediators of nonadrenergic, noncholinergic inhibition in the proximal, middle and distal regions of rat colon.大鼠结肠近端、中部和远端非肾上腺素能、非胆碱能抑制的介质
Br J Pharmacol. 1993 Feb;108(2):348-55. doi: 10.1111/j.1476-5381.1993.tb12808.x.
9
Inhibition of rat colon contractility by prostacyclin (IP-) receptor agonists: involvement of NANC neurotransmission.前列环素（IP-）受体激动剂对大鼠结肠收缩性的抑制作用：非肾上腺素能非胆碱能神经传递的参与
Br J Pharmacol. 1995 May;115(1):163-71. doi: 10.1111/j.1476-5381.1995.tb16334.x.
10
Effects of peptidases on non-adrenergic, non-cholinergic inhibitory responses of tracheal smooth muscle: a comparison with effects on VIP- and PHI-induced relaxation.肽酶对气管平滑肌非肾上腺素能、非胆碱能抑制反应的影响：与对血管活性肠肽和胰高血糖素样肽诱导的舒张作用的比较
Br J Pharmacol. 1989 Mar;96(3):521-6. doi: 10.1111/j.1476-5381.1989.tb11848.x.
本文引用的文献
1
A sensitive method for the assay of 5-hydroxytryptamine.一种测定5-羟色胺的灵敏方法。
Br J Pharmacol Chemother. 1957 Sep;12(3):344-9. doi: 10.1111/j.1476-5381.1957.tb00146.x.
2
Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.血管活性肠肽：犬胃肌层黏膜中的一种假定递质。
J Physiol. 1983 Aug;341:641-54. doi: 10.1113/jphysiol.1983.sp014830.
3
Pharmacological characterization of the postjunctional beta-adrenoceptors in the rat gastric fundus.
Eur J Pharmacol. 1984 Oct 30;106(1):1-9. doi: 10.1016/0014-2999(84)90671-x.
4
Pharmacological characterization of alpha adrenoceptors in the rat gastric fundus.大鼠胃底α肾上腺素能受体的药理学特性
J Pharmacol Exp Ther. 1984 Nov;231(2):404-10.
5
A novel non-adrenergic, non-cholinergic nerve-mediated relaxation of the pig bladder neck: an examination of possible neurotransmitter candidates.
Eur J Pharmacol. 1984 Apr 6;99(4):287-93. doi: 10.1016/0014-2999(84)90135-3.
6
Evidence against vasoactive intestinal polypeptide being the non-adrenergic, non-cholinergic inhibitory transmitter released from nerves supplying the smooth muscle of the guinea-pig taenia coli.关于血管活性肠肽并非从豚鼠结肠带平滑肌神经释放的非肾上腺素能、非胆碱能抑制性递质的证据。
Eur J Pharmacol. 1980 Oct 17;67(2-3):255-64. doi: 10.1016/0014-2999(80)90506-3.
7
Mechanism of action of chymotrypsin on plain muscle.
Br J Pharmacol Chemother. 1966 May;27(1):120-30. doi: 10.1111/j.1476-5381.1966.tb01647.x.
8
Effect of alpha-chymotrypsin on the nonadrenergic noncholinergic inhibitory system in cat airways.α-糜蛋白酶对猫气道非肾上腺素能非胆碱能抑制系统的作用。
Eur J Pharmacol. 1985 Aug 7;114(1):75-8. doi: 10.1016/0014-2999(85)90523-0.
10
Further evidence for involvement of adenosine-5'-triphosphate in non-adrenergic non-cholinergic relaxation of the isolated rat duodenum.三磷酸腺苷参与离体大鼠十二指肠非肾上腺素能非胆碱能舒张作用的进一步证据。
Eur J Pharmacol. 1985 Jul 31;113(3):399-408. doi: 10.1016/0014-2999(85)90088-3.
Zotero 插件