一种来自蛹虫草的新型核糖核苷酸。

A new ribonucleotide from Cordyceps militaris.

作者信息

Sun Jinfeng, Jin Mei, Zhou Wei, Diao Shengbao, Zhou Yi, Li Shuaishuai, Wang Xin, Pan Shuai, Jin Xuejun, Li Gao

机构信息

a Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education , Yanbian University College of Pharmacy , Yanji , P. R. China.

b Department of Pharmacy, Postdoctoral Research Station , Yanbian University Hospital , Yanji , P. R. China.

出版信息

Nat Prod Res. 2017 Nov;31(21):2537-2543. doi: 10.1080/14786419.2017.1323210. Epub 2017 May 15.

DOI:10.1080/14786419.2017.1323210

PMID:28503945

Abstract

One new ribonucleotide, 5'-(3''-deoxy-β-D-ribofuranosyl)-3'-deoxyadenosine (1), and 14 known compounds (2-15) were isolated from an ethanol extract of Cordyceps militaris. The chemical structures of these compounds were determined from 1D and 2D NMR (H-H COSY, HMBC, HMQC and NOESY) and HR-ESI-MS spectra, and results were compared with data from the literature. The effects of all isolated compounds were measured on NF-κB activation, with compound 2 exhibiting significant inhibitory activity against TNF-α-induced NF-κB reporter gene expression in HeLa cells from 3 to 100 μM.

摘要

从蛹虫草乙醇提取物中分离出一种新的核糖核苷酸，5'-(3''-脱氧-β-D-呋喃核糖基)-3'-脱氧腺苷(1)，以及14种已知化合物(2 - 15)。通过一维和二维核磁共振(H-H COSY、HMBC、HMQC和NOESY)以及高分辨电喷雾电离质谱(HR-ESI-MS)光谱确定了这些化合物的化学结构，并将结果与文献数据进行了比较。测定了所有分离化合物对NF-κB激活的影响，化合物2在3至100μM浓度下对HeLa细胞中TNF-α诱导的NF-κB报告基因表达表现出显著的抑制活性。