菠萝皮羧甲基纤维素/聚乙烯醇/介孔硅 SBA-15 水凝胶复合材料用于木瓜蛋白酶固定化。

Pineapple peel carboxymethyl cellulose/polyvinyl alcohol/mesoporous silica SBA-15 hydrogel composites for papain immobilization.

机构信息

School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.

Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.

出版信息

Carbohydr Polym. 2017 Aug 1;169:504-514. doi: 10.1016/j.carbpol.2017.04.057. Epub 2017 Apr 23.

DOI:10.1016/j.carbpol.2017.04.057

PMID:28504174

Abstract

Hydrogel composites based on pineapple peel carboxymethyl cellulose, polyvinyl alcohol and mesoporous silica SBA-15 were synthesized by an eco-friendly method of repeated freeze-thaw cycles for the application of papain immobilization. The experiment was optimized to obtain an efficient papain immobilization carrier. Simultaneously the immobilization conditions, including enzyme concentration, pH, crosslinker concentration and cross-linking time were optimized. The immobilized papain had maximum activity at low reaction temperature of 40°C and showed pH-sensitivity by exhibiting a rapid decrease of activity within a narrow range from pH 7.0 to pH 7.5. Compared with the free papain, the immobilized papain revealed enhanced pH, thermal and storage stability. After 2h incubation at 80°C, the immobilized papain retained 56% of its initial activity while the free papain only retained 16%. After 10days of storage, 79% of the initial activity was retained for the immobilized papain while only 27% for the free papain.

摘要

基于菠萝皮羧甲基纤维素、聚乙烯醇和介孔硅 SBA-15 的水凝胶复合材料是通过反复冻融循环的环保方法合成的，用于木瓜蛋白酶的固定化。该实验经过优化，以获得高效的木瓜蛋白酶固定化载体。同时，优化了固定化条件，包括酶浓度、pH 值、交联剂浓度和交联时间。固定化木瓜蛋白酶在低反应温度 40°C 下具有最大活性，并表现出 pH 敏感性，即在 pH7.0 至 pH7.5 的狭窄范围内活性迅速下降。与游离木瓜蛋白酶相比，固定化木瓜蛋白酶显示出增强的 pH 值、热稳定性和储存稳定性。在 80°C 下孵育 2 小时后，固定化木瓜蛋白酶保留了初始活性的 56%，而游离木瓜蛋白酶仅保留了 16%。储存 10 天后，固定化木瓜蛋白酶保留了初始活性的 79%，而游离木瓜蛋白酶仅保留了 27%。