Kang Jung-Mi, Lee Jinyoung, Kim Tae Im, Koh Eun-Ha, Kim Tong-Soo, Sohn Woon-Mok, Na Byoung-Kuk
Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Korea.
BK21Plus Team for Anti-aging Biotechnology and Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea.
Korean J Parasitol. 2017 Apr;55(2):159-165. doi: 10.3347/kjp.2017.55.2.159. Epub 2017 Apr 30.
Vivax malaria reemerged in Korea in 1993 and the outbreak has been continued with fluctuating numbers of annual indigenous cases. Understanding the nature of the genetic population of circulating in Korea is beneficial for the knowledge of the nationwide parasite heterogeneity and in the implementation of malaria control programs in the country. Previously, we analyzed polymorphic nature of merozoite surface protein-1 (MSP-1) and MSP-3α in Korean population and identified the Korean population has been diversifying rapidly, with the appearance of parasites with new genetic subtypes, despite the recent reduction of the disease incidence. In the present study, we developed simple PCR-RFLP methods for rapid subtyping of MSP-1 and MSP-3α of Korean isolates. These PCR-RFLP methods were able to easily distinguish each subtype of Korean MSP-1 and MSP-3α with high accuracy. The PCR-RFLP subtyping methods developed here would be easily applied to massive epidemiological studies for molecular surveillance to understand genetic population of and to supervise the genetic variation of the parasite circulating in Korea.
间日疟于1993年在韩国再次出现，疫情持续，每年本土病例数波动。了解韩国流行的间日疟原虫遗传群体的性质，有助于了解全国寄生虫的异质性，并有助于在该国实施疟疾控制计划。此前，我们分析了韩国人群中裂殖子表面蛋白-1（MSP-1）和MSP-3α的多态性，发现尽管近期疾病发病率有所下降，但韩国人群仍在迅速分化，出现了具有新遗传亚型的寄生虫。在本研究中，我们开发了简单的PCR-RFLP方法，用于快速鉴定韩国分离株的MSP-1和MSP-3α亚型。这些PCR-RFLP方法能够轻松、准确地区分韩国MSP-1和MSP-3α的各亚型。这里开发的PCR-RFLP亚型分析方法将很容易应用于大规模流行病学研究，用于分子监测，以了解韩国间日疟原虫的遗传群体，并监测在韩国流行的寄生虫的遗传变异。
