Labadie-Bracho Mergiory Y, Adhin Malti R
"Prof. Dr. Paul C. Flu" Institute for Biomedical Sciences Kernkampweg 5, Paramaribo, Suriname.
Anton de Kom Universiteit van Suriname, Faculty of Medical Sciences, Department of Biochemistry Kernkampweg 5, Paramaribo, Suriname.
Int J Mol Epidemiol Genet. 2021 Feb 15;12(1):9-15. eCollection 2021.
Suriname is on track to eliminate local malaria transmission. malaria reemerged in March and September 2019 in the Amerindian village Palumeu, free of malaria for two years and concurrently, a case was reported in another village Alalaparoe. The outbreaks were contained through targeted interventions including Mass Drug Administration (MDA). Molecular outbreak analysis was performed on 23 dried blood spots (DBS) using combined polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) with F2 and as polymorphic marker genes. Independent controls substantiated the discriminating capacities of the utilized PCR-RFLP method. All isolates from the first and second Palumeu outbreak shared a distinctive haplotype presuming single clonal lineage. An imported case probably triggered the first outbreak, while a delayed episode, prompted by withdrawal of drug pressure at the end of the prophylactic MDA, was suggested as source of the second outbreak. A diverging variant was demonstrated in Alalaparoe, implicating an infection from a different source. PCR-RFLP proved to be a useful molecular tool for outbreak management in low endemic malaria settings.
苏里南正朝着消除本地疟疾传播的目标迈进。2019年3月和9月，疟疾在美洲印第安人村落帕卢梅乌再度出现，该村落此前已两年无疟，与此同时，另一个村落阿拉拉帕罗埃也报告了一例疟疾病例。通过包括大规模药物管理（MDA）在内的有针对性干预措施，疫情得到了控制。使用联合聚合酶链反应/限制性片段长度多态性（PCR-RFLP），以F2和 作为多态性标记基因，对23个干血斑（DBS）进行了分子疫情分析。独立对照证实了所采用的PCR-RFLP方法的鉴别能力。帕卢梅乌第一次和第二次疫情的所有分离株都共享一种独特的单倍型，推测为单一克隆谱系。一例输入性病例可能引发了第一次疫情，而第二次疫情的源头被认为是预防性MDA结束时药物压力解除引发的延迟发作。在阿拉拉帕罗埃发现了一个不同的变体，这意味着感染来自不同的源头。PCR-RFLP被证明是在低流行疟疾环境中进行疫情管理的一种有用的分子工具。
