Krause J, Merz J
Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, TU Dortmund University, D-44227 Dortmund, Germany.
Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering, TU Dortmund University, D-44227 Dortmund, Germany.
J Chromatogr A. 2017 Jun 30;1504:64-70. doi: 10.1016/j.chroma.2017.05.006. Epub 2017 May 4.
Recently the Centrifugal Partition Chromatography (CPC) device is investigated as a reactor for biocatalytic reactions, as it enables biocatalyst immobilization without solid support and continuous operation of biphasic reaction systems. However, a detailed determination of the enzymes behavior in the CPC reactor and comparison to a classical stirred tank reactor (STR) for biphasic enzyme catalysis is not shown yet. In this study, the performance of an enzymatic biphasic hydrolysis reaction using lipase from Candida rugosa is systematically evaluated using a STR. The results are compared to different experiments conducted in the CPC reactor and used to evaluate the reaction performance in each. The same characteristics and limitations were observed in STR and CPC. At all states the CPC provided a similar reaction performance. However, the reaction in the CPC runs faster into limitations and was not easily scalable due to complex effects of the flow pattern. Although the enzyme was immobilized successfully and the activity of the lipase was preserved during CPC operation optimizations are needed to make the CPC reactor more competitive. For instance, scaling up the chamber geometry seems to be mandatory to increase the reaction performance, which may promote this reactor concept as an alternative to common devices for continuous biphasic biocatalytic reactions.
最近，离心分配色谱（CPC）装置被作为生物催化反应的反应器进行研究，因为它能够在无固体载体的情况下固定生物催化剂，并实现双相反应体系的连续运行。然而，尚未对CPC反应器中酶的行为进行详细测定，也未将其与用于双相酶催化的经典搅拌釜反应器（STR）进行比较。在本研究中，使用STR系统评估了皱褶假丝酵母脂肪酶催化的双相水解反应性能。将结果与在CPC反应器中进行的不同实验进行比较，并用于评估每个反应器的反应性能。在STR和CPC中观察到相同的特性和局限性。在所有状态下，CPC都具有相似的反应性能。然而，CPC中的反应更快达到极限，并且由于流动模式的复杂影响而不易放大。尽管酶已成功固定，且在CPC操作过程中脂肪酶的活性得以保留，但仍需要进行优化以使CPC反应器更具竞争力。例如，扩大腔室几何尺寸似乎是提高反应性能的必要条件，这可能会推动这种反应器概念成为连续双相生物催化反应常用装置的替代方案。
