Salvi Harshada M, Kamble Manoj P, Yadav Ganapati D
Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400019, India.
Appl Biochem Biotechnol. 2018 Feb;184(2):630-643. doi: 10.1007/s12010-017-2572-7. Epub 2017 Aug 23.
With increasing demand for perfumes, flavors, beverages, and pharmaceuticals, the various associated industries are resorting to different approaches to enhance yields of desired compounds. The use of fixed-bed biocatalytic reactors in some of the processes for making fine chemicals will be of great value because the reaction times could be reduced substantially as well as high conversion and yields obtained. In the current study, a continuous-flow packed-bed reactor of immobilized Candida antarctica lipase B (Novozym 435) was employed for synthesis of various geraniol esters. Optimization of process parameters such as biocatalyst screening, effect of solvent, mole ratio, temperature and acyl donors was studied in a continuous-flow packed-bed reactor. Maximum conversion of ~ 87% of geranyl propionate was achieved in 15 min residence time at 70 °C using geraniol and propionic acid with a 1:1 mol ratio. Novozym 435 was found to be the most active and stable biocatalyst among all tested. Ternary complex mechanism with propionic acid inhibition was found to fit the data.
随着对香水、香料、饮料和药品的需求不断增加，各个相关行业都在采用不同方法来提高所需化合物的产量。在一些精细化学品生产过程中使用固定床生物催化反应器具有重要价值，因为这可以大幅缩短反应时间，并实现高转化率和高产率。在本研究中，使用固定化南极假丝酵母脂肪酶B（诺维信435）的连续流填充床反应器来合成各种香叶醇酯。在连续流填充床反应器中研究了生物催化剂筛选、溶剂效应、摩尔比、温度和酰基供体等工艺参数的优化。在70℃下，使用摩尔比为1:1的香叶醇和丙酸，停留时间为15分钟时，丙酸香叶酯的最大转化率达到约87%。在所有测试的生物催化剂中，诺维信435被发现是活性最高且最稳定的。发现丙酸抑制的三元复合机制符合数据。
