Yang Hao, Gao Hongyu, Xie Xianju, Wang Hongmei, Li Xiaowei, Qiao Qingchen, Ma Yansong, Bai Yuxing
Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
J Oral Microbiol. 2025 Jun 30;17(1):2522421. doi: 10.1080/20002297.2025.2522421. eCollection 2025.
White spot lesions (WSLs) are a common complication of orthodontic treatment. However, the cariogenic discrepancy in the supragingival microbiome between demineralized and non-demineralized surfaces and the influence of associated with WSLs remain unexplored. This study investigated the changes in supragingival microbiome of orthodontic adolescents with WSLs, encompassing both demineralized and non-demineralized sites, and explored colonization in these patients. Supragingival plaques were collected from 29 orthodontic adolescents with WSLs (categorized into demineralized and non-demineralized groups based on the presence/absence of demineralization at sampling sites) and 23 healthy orthodontic adolescents. Supragingival microbiome composition was evaluated using 16S rRNA sequencing, and colonization was identified using fungal culture methods. The supragingival microbiome on non-demineralized surfaces showed intermediate cariogenic potential between demineralized and healthy states, but closer to the demineralized state. exhibited a propensity for colonization in WSLs patients without site-specificity. influenced bacterial composition, with significantly enriched on the demineralized surfaces of -positive patients. In orthodontic adolescents with WSLs, non-demineralized surfaces showed microbiome shifts, necessitating interventions to promote a healthy microbiome. can impact microbiome composition and potentially contribute to WSLs pathogenesis.
白斑病变（WSLs）是正畸治疗常见的并发症。然而，脱矿表面与非脱矿表面龈上微生物群的致龋差异以及与WSLs相关的影响仍未得到探索。本研究调查了患有WSLs的正畸青少年龈上微生物群的变化，包括脱矿部位和非脱矿部位，并探索了这些患者中的真菌定植情况。从29名患有WSLs的正畸青少年（根据采样部位是否存在脱矿分为脱矿组和非脱矿组）和23名健康的正畸青少年中收集龈上菌斑。使用16S rRNA测序评估龈上微生物群组成，并使用真菌培养方法鉴定真菌定植情况。非脱矿表面的龈上微生物群的致龋潜力介于脱矿状态和健康状态之间，但更接近脱矿状态。真菌在WSLs患者中表现出定植倾向，且无部位特异性。真菌影响细菌组成，在白色念珠菌阳性患者的脱矿表面显著富集。在患有WSLs的正畸青少年中，非脱矿表面显示出微生物群的变化，需要采取干预措施来促进健康的微生物群。真菌可影响微生物群组成，并可能导致WSLs的发病机制。
