Gill Alexander
Health Canada, Bureau of Microbial Hazards, OttawaON, Canada.
Front Microbiol. 2017 May 1;8:777. doi: 10.3389/fmicb.2017.00777. eCollection 2017.
Culture-based and genomics methods provide different insights into the nature and behavior of bacteria. Maximizing the usefulness of both approaches requires recognizing their limitations and employing them appropriately. Genomic analysis excels at identifying bacteria and establishing the relatedness of isolates. Culture-based methods remain necessary for detection and enumeration, to determine viability, and to validate phenotype predictions made on the bias of genomic analysis. The purpose of this short paper is to discuss the application of culture-based analysis and genomics to the questions food microbiologists routinely need to ask regarding bacteria to ensure the safety of food and its economic production and distribution. To address these issues appropriate tools are required for the detection and enumeration of specific bacterial populations and the characterization of isolates for, identification, phylogenetics, and phenotype prediction.
基于培养的方法和基因组学方法为细菌的本质和行为提供了不同的见解。要使这两种方法都发挥最大效用，就需要认识到它们的局限性并恰当地运用它们。基因组分析在识别细菌和确定分离株的亲缘关系方面表现出色。基于培养的方法对于检测和计数、确定生存能力以及验证基于基因组分析做出的表型预测仍然是必要的。本短文的目的是讨论基于培养的分析和基因组学在食品微生物学家日常需要询问的有关细菌的问题中的应用，以确保食品的安全及其经济生产和分销。为了解决这些问题，需要适当的工具来检测和计数特定细菌群体，并对分离株进行鉴定、系统发育分析和表型预测。
