Sicinska Paulina, Bukowska Bozena, Pajak Aneta, Koceva-Chyla Aneta, Pietras Tadeusz, Nizinkowski Piotr, Gorski Paweł, Koter-Michalak Maria
Department of Environmental Pollution Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Department of Pneumology and Allergology, Norbert Barlicki Memorial University Hospital, Medical University of Lodz, Lodz, Poland.
Arch Med Sci. 2017 Apr 1;13(3):645-651. doi: 10.5114/aoms.2016.60760. Epub 2016 Jun 20.
Butyrylcholinesterase (BChE) is involved in the metabolism of endogenous lipids and xenobiotics, such as esters of carboxylic or phosphoric acids. Butyrylcholinesterase activity is associated with both inflammation and oxidative stress. Changes in the activity of this enzyme have been observed in various diseases such as liver cirrhosis, diabetes, neurodegenerative disease and others.
The study involved 30 patients with chronic obstructive pulmonary disease (COPD) and 18 healthy subjects. The COPD patients were divided according to the severity of the disease by applying the classification of COPD based on GOLD standards for forced expiratory volume in 1 s (FEV) and the FEV/forced expiratory volume (FVC) ratio. The control group comprised blood samples collected from healthy subjects without concomitant diseases related to the respiratory system. Butyrylcholinesterase activity, lipid peroxidation and total antioxidant capacity (TAC) were determined in the blood plasma.
A significant ( < 0.05) decrease in the activity of BChE, associated with an increase in lipid peroxidation and a decrease in the total antioxidant capacity, was observed in blood plasma of patients with chronic obstructive pulmonary disease.
The study shows for the first time that activity of BChE in the blood plasma of patients diagnosed with chronic obstructive pulmonary disease is considerably reduced compared with healthy subjects. These changes were accompanied by a decrease of TAC and an increase of lipid peroxidation, which suggests that they may be related to the oxidative stress induced by COPD disease.
丁酰胆碱酯酶（BChE）参与内源性脂质和外源性物质（如羧酸或磷酸酯）的代谢。丁酰胆碱酯酶活性与炎症和氧化应激均有关。在肝硬化、糖尿病、神经退行性疾病等多种疾病中均观察到该酶活性的变化。
该研究纳入了30例慢性阻塞性肺疾病（COPD）患者和18名健康受试者。根据基于1秒用力呼气容积（FEV）和FEV/用力肺活量（FVC）比值的GOLD标准对COPD患者进行疾病严重程度分类。对照组包括从无呼吸系统相关伴随疾病的健康受试者采集的血样。测定血浆中的丁酰胆碱酯酶活性、脂质过氧化和总抗氧化能力（TAC）。
在慢性阻塞性肺疾病患者的血浆中观察到丁酰胆碱酯酶活性显著降低（<0.05），同时脂质过氧化增加，总抗氧化能力降低。
该研究首次表明，与健康受试者相比，被诊断为慢性阻塞性肺疾病的患者血浆中丁酰胆碱酯酶活性显著降低。这些变化伴随着TAC的降低和脂质过氧化的增加，这表明它们可能与COPD疾病诱导的氧化应激有关。
