龋齿患者唾液免疫球蛋白基因表达
Salivary Immunoglobulin Gene Expression in Patients with Caries.
作者信息
Santín Gema Regina Guadarrama, Salgado Angel Visoso, Bastida Norma Margarita Montiel, Gómez Isaías de la Rosa, Benítez Jonnathan Guadalupe Santillán, Zerón Hugo Mendieta
机构信息
Laboratory of Molecular Biology, Medical Sciences Research Center (CICMED), Autonomous University of the State of Mexico (UAEMex), Jesús Carranza 205, Col. Universidad, C.P. 50130, Toluca, México, Mexico.
出版信息
Open Access Maced J Med Sci. 2017 Mar 3;5(2):236-243. doi: 10.3889/oamjms.2017.028. eCollection 2017 Apr 15.
BACKGROUND
Immunoglobulins mediate the host's humoral immune response are expressed in saliva.
AIM
To quantify the , , and gene expression in the saliva of Mexican patients with caries in mixed and permanent dentition.
SUBJECTS AND METHODS
This was a comparative cross-sectional study. mRNA was isolated from 200 μL of saliva following the RNA III Tissue Fresh-frozen protocol of the MagNA Pure LC Instrument 2.0 (Roche Diagnostics GmbH, Nederland BV) and the , and were quantified through TaqMan Assays.
RESULTS
One hundred individuals, 50 with mixed dentition and 50 with permanent dentition, were included in the study. Statistically, it was found a significant difference (p = 0.025) in the IgG () expression between the studied groups.
CONCLUSION
Although we confirmed the existence of , and gene expression in saliva, only a significant difference in the expression of between the mixed dentition and permanent dentition was found.
背景
介导宿主体液免疫反应的免疫球蛋白在唾液中表达。
目的
量化墨西哥混合牙列和恒牙列龋病患者唾液中 、 和 基因的表达。
对象与方法
这是一项比较性横断面研究。按照MagNA Pure LC仪器2.0（罗氏诊断有限公司，荷兰分公司）的RNA III组织新鲜冷冻方案，从200 μL唾液中分离mRNA，并通过TaqMan分析法定量 、 和 。
结果
该研究纳入了100名个体，其中50名处于混合牙列，50名处于恒牙列。在统计学上，研究组之间的IgG（）表达存在显著差异（p = 0.025）。
结论
尽管我们证实了唾液中存在 、 和 基因表达，但仅发现混合牙列和恒牙列之间的 表达存在显著差异。
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