Yang Haoshu, Gu Wenpeng, Qiu Haiyan, Sun Guixiang, Liang Junrong, Li Kewei, Xiao Yuchun, Duan Ran, Jing Huaiqi, Wang Xin
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing, China.
Lianyungang Center of Disease Control and PreventionLianyungang, China.
Front Cell Infect Microbiol. 2017 May 1;7:158. doi: 10.3389/fcimb.2017.00158. eCollection 2017.
Pathogenic is widely distributed in China where the primary bio-serotypes are 3/O: 3 and 2/O: 9. Recently, the distribution of 2/O: 9 strains are being gradually replaced by 3/O: 3 strains where presently 3/O: 3 strains are the major pathogenic in China. To identify the growth conditions and cytokines induced by and providing some clues for this shift, we performed competitive growth and for these two bio-serotype strains; and we also compared the cytokines induced by them in infected BALB/C mice. We found 2/O: 9 strains grew more , while 3/O: 3 strains grew more regardless of using single cultures or mixed cultures. The cytokines induced by the two strains were similar: interleukin-6 (IL-6), IL-9, IL-13, granulocyte colony-stimulating factor (G-CSF), chemokines (KC), monocyte chemotactic protein 1 (MCP-1), macrophage inflammation protein-1α (MIP-1α), tumor necrosis factor-α (TNF-α), and RANTES were statistically up-regulated upon activation of normal T cells compared to the control. The cytokine values were higher in mixed infections than in single infections except for IL-6, G-CSF, and KC. The data illustrated the different growth of pathogenic bio-serotype 3/O: 3 and 2/O: 9 and , and the cytokine changes induced by the two strains in infected BALB/C mice. The growth comparisons of two strains maybe reflect the higher pathogenic ability or resistance to host immune response for bio-serotype 3/O: 3 and maybe it as one of the reason for bacteria shift.
致病性[细菌名称]在中国广泛分布，其主要生物血清型为3/O:3和2/O:9。最近，2/O:9菌株的分布正逐渐被3/O:3菌株所取代，目前3/O:3菌株是中国主要的致病[细菌名称]。为了确定[细菌名称]诱导的生长条件和细胞因子，并为这种转变提供一些线索，我们对这两种生物血清型菌株进行了竞争性生长[实验名称]和[实验名称]；我们还比较了它们在感染BALB/C小鼠中诱导的细胞因子。我们发现，无论使用单一培养还是混合培养，2/O:9菌株生长得更快，而3/O:3菌株生长得更慢。两种菌株诱导的细胞因子相似：与对照相比，正常T细胞激活后，白细胞介素-6（IL-6）、IL-9、IL-13、粒细胞集落刺激因子（G-CSF）、趋化因子（KC）、单核细胞趋化蛋白1（MCP-1）、巨噬细胞炎症蛋白-1α（MIP-1α）、肿瘤坏死因子-α（TNF-α）和调节激活正常T细胞表达和分泌的趋化因子（RANTES）在统计学上上调。除IL-6、G-CSF和KC外，混合感染中的细胞因子值高于单一感染。数据说明了致病性[细菌名称]生物血清型3/O:3和2/O:9的不同生长[情况]，以及两种菌株在感染BALB/C小鼠中诱导的细胞因子变化。两种菌株的生长比较可能反映了生物血清型3/O:3的更高致病能力或对宿主免疫反应的抵抗力，这可能是细菌转变的原因之一。
