Schasfoort E M, De Bruin L A, Korf J
Department of Biological Psychiatry, Psychiatric University Clinic, Groningen, The Netherlands.
Brain Res. 1988 Dec 13;475(1):58-63. doi: 10.1016/0006-8993(88)90198-9.
Lactography is a novel technique that allows the continuous on-line registration of brain extracellular lactate in the freely behaving animal and that is based on a fluorimetric enzymatic assay of brain dialysates. Electroconvulsive shock, activation of the glutamate receptor (NMDA-type) and mild stress (immobilization, cold stress or handling) result in transient increases in the efflux of lactate from the rat hippocampus. The increase following immobilization stress was attenuated by the NMDA-receptor antagonist 2-amino-5-phosphopentanoic acid and after several pre-exposures to this stressor. These experiments suggest that mild stress activates glutamatergic neurons, which may be less after habituation to stress.
乳腺造影术是一种新颖的技术，它能够在自由活动的动物体内连续在线记录脑细胞外乳酸水平，且该技术基于对脑透析液的荧光酶测定法。电休克、谷氨酸受体（NMDA型）激活以及轻度应激（固定、冷应激或处理）会导致大鼠海马体乳酸外流短暂增加。固定应激后的增加被NMDA受体拮抗剂2-氨基-5-磷酸戊酸减弱，并且在多次预先暴露于该应激源后也会减弱。这些实验表明，轻度应激会激活谷氨酸能神经元，而在适应应激后这种激活可能会减少。
