Krugers H J, Jaarsma D, Korf J
Department of Biological Psychiatry, University of Groningen, The Netherlands.
J Neurochem. 1992 Mar;58(3):826-30. doi: 10.1111/j.1471-4159.1992.tb09331.x.
The role of the entorhinal cortex and the adrenal gland in rat hippocampal lactate formation was assessed during and after a short-lasting immobilization stress and electroconvulsive shock (ECS). Extracellular lactate was measured on-line using microdialysis and enzyme reactions (a technique named lactography); in some rats, unilateral lesions of the entorhinal cortex were made or the bilateral adrenal glands were removed. The stress-evoked increase in hippocampus lactate was not altered either ipsi- or contralateral to an entorhinal cortex lesion. The response to ECS was attenuated only in the hippocampus ipsilateral to the entorhinal cortex lesion. Removal of bilateral adrenal glands caused some delay in the increase in hippocampal lactate after ECS and a major reduction in the stress-evoked lactate response. These results indicate that (1) the entorhinal cortex is activated by ECS, thereby activating hippocampal lactate efflux and presumably metabolism, and (2) the adrenal gland is essential in the response to stress and, to a minor extent, in the ECS-altered hippocampal metabolism.
在短暂的固定应激和电惊厥休克（ECS）期间及之后，评估了大鼠内嗅皮质和肾上腺在海马乳酸生成中的作用。使用微透析和酶反应（一种名为乳酸成像的技术）在线测量细胞外乳酸；在一些大鼠中，对内嗅皮质进行单侧损伤或切除双侧肾上腺。内嗅皮质损伤同侧或对侧海马中，应激诱发的乳酸增加均未改变。仅在内嗅皮质损伤同侧的海马中，对ECS的反应减弱。切除双侧肾上腺会导致ECS后海马乳酸增加出现一些延迟，并使应激诱发的乳酸反应大幅降低。这些结果表明：（1）内嗅皮质被ECS激活，从而激活海马乳酸外流并可能激活代谢；（2）肾上腺在应激反应中至关重要，在较小程度上也参与ECS改变的海马代谢。
